- 中文名称
Notch Reporter BioAssay Kit (cAMP/PKA Pathway)
- 英文名字
- Notch Reporter BioAssay Kit (cAMP/PKA Pathway)
- 供应商
- USBiological
- 产品货号
- 171935
- 产品报价
- ￥1/500Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Notch Pathway Reporter BioAssay kit is designed for monitoring the activity of the Notch signaling pathway in cultured cells. The kit contains a transfection-ready expression vector forNOTCH1 that has a deletion of the entire extracellular domain (Notch1deltaE). Inside thecells, the NOTCH1 DE can be cleaved by upsilon-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity.Application:Monitor Notch signaling pathway activity.Screen activators or inhibitors of Notch signaling pathway.Study effects of RNAi or gene overexpression on the activity of Notch pathway.Components:Reporter (Component A), CSL (CBF1/RBP-Jkappa) 1x500ulNegative Control (Component B), 1x500ulNotch1deltaE (Component C), 1x250ulNegative Control Expression vector (Component D), 1x250ul Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Notch Reporter BioAssay Kit (cAMP/PKA Pathway) 该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Notch Reporter BioAssay Kit (cAMP/PKA Pathway) 产品后将其置于-20°C/-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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