The NF-kB Reporter BioAssay kit is designed for monitoring the activity of the NF-kB signaling pathway in the cultured cells. The kit contains transfection-ready NF-kB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-kB responsive element located upstream of a minimal promoter. The NF-kB reporter is premixed with constitutively-expressing Renilla luciferase vector, whichserves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity. Applications:Monitor NF-kB pathway activity.Screen activators or inhibitors of the NF-kB signaling pathway.Study effects of RNAi or gene overexpression on the activity of the NF-kBpathway.Components:Reporter (Component A), NF-kB luciferase reporter vector + constitutively expressing Renilla luciferase vector, 1x500ul (60ng DNA/ul)Negative Control Reporter (Component B), Non-inducible luciferase vector + constitutively expressing Renilla luciferase vector, 1x500ul (60ng DNA/ul)Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
