Components in this kit are prepared with pure chemicals according to our proprietary technology. QPCR SuperMix is a 2x concentration of premix reagent including Hot-start DNA polymerase and Eva Dye and specially formulated real time buffer designed for real-time PCR with intercalator format.Real–time or quantitative PCR (QPCR) allows quantification of DNA, cDNA, or RNA templates. QPCR is based on the detection of a fluorescent reporter molecule that increases as PCR products accumulate with each cycle of amplification. In Eva QPCR SuperMix, a superior green fluorescence DNA-binding dye is used for real-time detection and quantitation of DNA. The Eva QPCR SuperMix is a ready-to-use, 2x-concentrated master mix that contains all the reagents (except primers and templates) needed for running quantitative, real-time DNA detection assays, in the double-stranded DNA dye detection format. The passive reference dye ROX is included in a separate tube to make the Eva Supermix adaptable for many real-time QPCR platforms. A pair of human beta-actin primers is also included in the kit as a control. QPCR SuperMix contains United States Biological’s Taq polymerase with hot start capability. hot-start Taq polymerase improves PCR amplification reactions by decreasing nonspecific amplification and preventing primer-dimer formation. This enzyme is activated after an initial seven to ten minutes heating at 95°C. And the real-time PCR buffer is specially formulated to provide superior specificity and increase amplification efficiency. This SuperMix can amplify and detect a broad range of DNA or cDNA targets, including those are GC- or AT-rich.Kit Components:Eva QPCR SuperMixROX reference DyeHuman beta-actin control F/R primer pair (25x)Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
