Protein carbonyl groups are an important and immediate biomarker of oxidative stress. DNPH tagging of protein carbonyls has been one of the most common measures of oxidative stress. DNP hydrazones formed from the reaction are easily quantifiable at 375nm. Protein Carbonyl Content Assay Kit is designed to provide a simple and accurate method of quantifying carbonyls in protein samples. Using BSA as an example, a 1mg (~15nmol) sample has a detection limit of about 0.15nmol carbonyl, where BSA typically contains approximately 1-3nmol carbonyl/mg.Sample type:Variable (protein containing samples)Intended Use:Designed to provide a simple and accurate method of quantifying carbonyls in protein samples.Test Principle:Protein Carbonyl Content Assay Kit is designed to provide a simple and accurate method of quantifying carbonyls in protein samples. Using BSA as an example, a 1mg (~15nmol) sample has a detection limit of about 0.15nmol carbonyl, where BSA typically contains approximately 1-3nmol carbonyl/mg.Specificity:MammalianKit Components:DNPH Solution: 1x11ml 100% TCA Solution: 1x3ml 10% Streptozocin Solution: 1xqml 6M Guanidine Solution 96-Well Clear PlateStorage and Stability:Store powder at 4°C, liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
