Digitonin is a steroid glycoside from Digitalis purpurea. It belongs to the class of non-ionic detergents commonly used for solubilization of membrane proteins and permeabilization cellular and nuclear membranes. It permeabilizes plasma membranes of eukaryotic cells by complexing with membrane cholesterol and other unconjugated beta-hydroxysterols.Solubility:Warm H2O (~50mg/ml) or warm EtOH (~25mg/ml)Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
