- 中文名称
Phosphofructokinase (PFK), Mammalian, BioAssay Activity Assay Kit (Colorimetric)
- 英文名字
- Phosphofructokinase (PFK), Mammalian, BioAssay Activity Assay Kit (Colorimetric)
- 供应商
- USBiological
- 产品货号
- 167945
- 产品报价
- ￥1/100Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet). In humans, PFK deficiency causes glycogen storage disease, also called Tarui’s disease, which is characterized by exercise-induced muscle weakness and cramps. On the other hand, increased PFK activity contributes to cancer cell proliferation and tumorigenicity. Early detection of abnormal phosphofructokinase activity is crucial for diagnosis, prediction and therapeutic strategy. In Phosphofructokinase Activity Assay kit, PFK converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450nm. PFK activity assay is simple, robust, and rapid and can detect phosphofructokinase activity less than 1mU.Sample Type:Animal tissues, cell cultures. Intended Use:Measurement of phosphofructokinase activity in various tissues/cells. Analysis of glucose metabolism and cell signaling in various cell types. Screening anti-cancer drugs.Sensitivity:can detect PFK activity at <1mU.Kit Components:PFK assay buffer, 1x27mlPFK substrate (lyophilized), 1 vialATP (lyophilized), 1 vialPFK enzyme mix (lyophilized), 1 vialPFK developer (lyophilized), 1 vialNADH standard (lyophilized), 1 vialPositive control (lyophilized), 1 vialStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Phosphofructokinase (PFK), Mammalian, BioAssay Activity Assay Kit (Colorimetric)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Phosphofructokinase (PFK), Mammalian, BioAssay Activity Assay Kit (Colorimetric)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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