- 中文名称
Nesfatin BioAssay ELISA Kit (Human, Mouse, Rat)
- 英文名字
- Nesfatin BioAssay ELISA Kit (Human, Mouse, Rat)
- 供应商
- USBiological
- 产品货号
- 167918
- 产品报价
- ￥1/100Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Nesfatin Enzyme Immunoassay (EIA) BioAssay Kit is an in vitro quantitative assay for detecting Nesfatin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Nesfatin antibody, both biotinylated Nesfatin peptide and peptide standard or targeted peptide in samples interacts competitively with the Nesfatin antibody. Uncompeted (bound) biotinylated Nesfatin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Nesfatin peptide in the standard or samples. This is due to the competitive binding to Nesfatin antibody between biotinylated Nesfatin peptide and peptides in standard or samples. A standard curve of known concentration of Nesfatin peptide can be established and the concentration of Nesfatin peptide in the samples can be calculated accordingly. EIA-NES-1 detects 420aa Nesfatin protein. No other active isoforms have been reported. The minimum detectable concentration of Nesfatin is 147pg/ml or 3.09pM. Sample Type:Serum, plasma and cell culture media etc.Intended Use:The BioAssay kit is used for quantitative protein detection, establishing normal range and validation of antibody array results.Specificity:The capture antibody provided in this kit recognizes human, mouse and rat Nesfatin.Sensitivity:The intra-assay reproducibility is CV<10% and inter-assay is CV<12%. Range:The detection range for the kit is 0.1-1000ng/mlKit Components:Nesfatin Microplate (Item A), 1x96wellsWash Buffer Concentrate (Item B), 1x25mlStandard Nesfatin peptide (Item C), 2x vialsAssay Diluent A (Item D), 1x30mlAssay Diluent B (Item E), 1x15mlBiotinylated Nesfatin peptide (Item F), 2x vialsHRP-Streptavidin concentrate (Item G), 1x600ulTMB One-Step Substrate Reagent (Item H), 1x12mlStop Solution (Item I), 1x8mlPositive control (Item M), 1x vialAnti- Nesfatin polyclonal antibody (Item N), 2x vialsStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Nesfatin BioAssay ELISA Kit (Human, Mouse, Rat)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Nesfatin BioAssay ELISA Kit (Human, Mouse, Rat)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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