c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes. Sample Type:Cell and tissue lysates. Intended Use:The kit can be used to screen JNK activity relatively quickly for large numbers of samples.Test Principle:The JNK Activity screening BioAssay kit utilizes an N-terminal c-Jun (1-79) fusion protein bound to glutathion sepharose beads to selectively “pull down” JNK from cell lysates. After wash to remove nonspecifically bound proteins, the kinase reaction is then carried out in the presence of cold ATP. c-Jun phosphorylation is then measured by Western blot analysis using a phospho-c-Jun specific antibody.Kit Components:Kinase Extraction Buffer, 1x80mlc-Jun (1-79) Fusion Protein Beads, 1x800ulKinase Assay Buffer, 1x25mlATP, 10mM, 1x50ulPhospho-cJun Specific Antibody, 1x50ulStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
