- 中文名称
Interleukin 2, BioAssay ELISpot Kit (Primate) (IL-2)
- 英文名字
- Interleukin 2, BioAssay ELISpot Kit (Primate) (IL-2)
- 供应商
- USBiological
- 产品货号
- 167669
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Interleukin 2 (IL-2) was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has also been shown to stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. At the amino acid sequence level, there is approximately 60% - 90% similarity between species. Mature human IL-2 shows 65%, 67%, 72%, 78%, and 64% aa identity to mouse, rat, pig, cat, and cow IL-2, respectively.Intended Use:For the quantitative determination of the frequency of cells releasing primate IL-2.Test Principle:The enzyme-linked immunospot (ELISpot) assay was originally developed for the detection ofindividual B cells secreting antigen-specific antibodies (21, 22). This method has since beenadapted for the detection of individual cells secreting specific cytokines or other antigens(23, 24). ELISpot assays employ the quantitative sandwich enzyme-linked immunosorbentassay (ELISA) technique. A monoclonal antibody specific for primate IL-2 has been pre-coatedonto a PVDF (polyvinylidene difluoride)-backed microplate. Appropriately stimulated cells arepipetted into the wells and the microplate is placed into a humidified 37°C CO2 incubator for a specified period of time. During this incubation period, the immobilized antibody in the immediate vicinity of the secreting cells bind secreted IL-2. After washing away any cells and unbound substances, a biotinylated polyclonal antibody specific for primate IL-2 is added to the wells. Following a wash to remove any unbound biotinylated antibody, alkaline-phosphatase conjugated to streptavidin is added. Unbound enzyme is subsequently removed by washingand a substrate solution (BCIP/NBT) is added. A blue-black colored precipitate forms and appears as spots at the sites of cytokine localization, with each individual spot representing an individual IL-2 secreting cell. The spots can be counted with an automated ELISpot readersystem or manually using a stereomicroscope.Kit Components:1. Precoated Microplate, 1x96 wells2. Detection Antibody Concentrate, 1x150ul3. Streptavidin-AP Concentrate A, 1x150ul4. Dilution Buffer 1, 1x12ml5. DIlution Buffer 2, 1x12ml6. Wash Buffer Concentrate, 1x50ml7. BCIP/NBT Chromogen, 1x12ml8. Primate IL-2 Positive Control, 1x1vialStorage and Stability:Store components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Interleukin 2, BioAssay ELISpot Kit (Primate) (IL-2)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interleukin 2, BioAssay ELISpot Kit (Primate) (IL-2)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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