- 中文名称
dsDNA IgG, Human, BioAssay ELISA Kit
- 英文名字
- dsDNA IgG, Human, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- 167620
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Anti-dsDNA IgG test is used for initial diagnosis of Systemic Lupus Erythematosus (SLE) and for diagnosis of SLE different diseases. Besides the determination of high titers of antinuclear antibody (ANA), the determination of autoantibodies against dsDNA is one of the ACR criteria (American College of Rheumatology) for the diagnosis of Systemic Lupus Erythematosus (SLE). The determination of the concentration of antibodies can be used to monitor treatment success and predict possible attacks of the disease (SLE) Intended Use:Anti dsDNA IgG kit is an indirect solid phase enzyme immunometric assay (ELISA) designed for the quantitative measurement of IgG class antibodies directed against dsDNA in human serum or plasma. This kit is intended for laboratory use only.Specificity:Comparison test against a commercial reference kit, performed on 100 serum (50 of them positive serum and 50 negative serum) showed a >99% specificity. Sensitivity:Comparison test against a commercial reference kit, performed on 100 serum (50 of them positive serum and 50 negative serum) showed a >99% sensitivity. Detection Limit:135IU/mlTest Principle:Anti-dsDNA IgG test is based on the binding of serum or plasma IgG antibodies on dsDNA coated on the microplates. The antibodies in calibrators, controls or prediluted patient samples bind into the inner surface of the wells. After a 30 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components. An anti-human-IgG horseradish peroxidase conjugate solution recognizes the IgG class antibodies bound to the immobilized dsDNA antigens. After a 30 minutes incubation any excess of enzyme conjugate, which is not specifically bound, is washed away with wash buffer. A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color development is stopped by adding the stop solution. The solution color changes into yellow. The amount of color is directly proportional to the concentration of the anti-dsDNA IgG antibodies present in the original sample. The concentration of the anti dsDNA IgG antibodies in the sample are calculated through a calibration curve.Kit Components:167620A: Microtiter Strips, 1x96wells. Coated with dsDNA.167620B: Standard 0IU/ml, 1x1.2ml167620C: Standard 12.5IU/ml, 1x1.2ml167620D: Standard 25IU/ml, 1x1.2ml167620E: Standard 50IU/ml, 1x1.2ml167620F: Standard 200IU/ml, 1x1.2ml167620G: Negative Control, 1x1.2ml167620H: Positive Control, 1x1.2ml167620J: IgG (HRP), 1x15ml167620K: Sample Diluent, 1x100ml167620L: TMB, 1x15ml167620M: Wash Solution, 10X, 1x50ml167620N: Stop Solution (H2SO4): 1x15mlStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对dsDNA IgG, Human, BioAssay ELISA Kit 该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到dsDNA IgG, Human, BioAssay ELISA Kit 产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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