- 中文名称
Vitamin B12 (VB12, Cyanocobalamin) BioAssay ELISA Kit (Human)
- 英文名字
- Vitamin B12 (VB12, Cyanocobalamin) BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- 167568
- 产品报价
- ￥1/96 Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Vitamin B12 ELISA Kit is an enzyme immunoassay (ELISA) for the quantitative detection of Vitamin B12 concentration in human serum. The Vitamin B12 ELISA test is for in vitro diagnostic use only. Sensitivity: 70.13pg/mlTest Principle:Delayed Competitive Enzyme Immunoassay (TYPE 9): The essential reagents required for a enzyme immunoassay include antibody, enzyme-antigen conjugate and native antigen. Upon mixing the biotinylated antibody with a serum containing the antigen, a reaction results between the antigen and the antibody.After a short incubation, the enzyme conjugate is added (This delayed addition permits and increase in sensitivity for low concentration samples). Upon the addition of the enzyme conjugate, competition reaction results between the enzyme analogand the antigen in the sample for a limited number of antibody binging sites (not consumed in the first incubation).A simultaneous reaction between the biotin attachedto the antibody and the streptavidin immobilized on the microwell occurs. This effects the separation of the antibody bound fraction after decantation or aspiration.The enzyme activity in the antibody bound fraction is inversely proportional to the native antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.Kit Components:167568A: Microtiter Plate 1x96 wells 167568B: Standard 0pg/ml 1x1ml167568C: Standard 100pg/ml 1x1ml167568D: Standard 200pg/ml 1x1ml167568E: Standard 400pg/ml 1x1ml167568F: Standard 1000pg/ml 1x1ml167568G: Standard 2000pg/ml 1x1ml167568H: Vitamin B12 (HRP): 1x7ml 167568J: Vitamin B12 (Biotin): 1x7ml167568K: Stop Solution (0.5M H2SO4):1x8ml167568L: Wash Solution, (PBS + Tween-20): 1x20ml 167568M: Substrate Solution (TMB, H2O2): 1x12ml167568N: Releasing Agent: 1x14ml 167568P: Stabilizing Agent: 1x700ul 167568Q: Neutralizing Buffer: 1x7ml The calibrators can be expressed in molar concentrations (pM/L) by multiplying by 0.738. For example: 100pg/ml x 0.738 = 73.8pM/L.Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Vitamin B12 (VB12, Cyanocobalamin) BioAssay ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Vitamin B12 (VB12, Cyanocobalamin) BioAssay ELISA Kit (Human)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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