- 中文名称
Treponema pallidum IgM (Syphilis) BioAssay ELISA Kit
- 英文名字
- Treponema pallidum IgM (Syphilis) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- 167567
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Intended Use: The Syphilis TPA-Treponema pallidum IgM ELISA kit provides materials for the qualitative and semi quantitative determination of IgM-class antibodies to Treponema pallidum in serum. This is intended for in vitro diagnostic use onlyPrinciple of the Assay:The Treponema pallidum IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Sample Diluent is used to dilute patient samples, and then incubating with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to exclude competitive inhibition from specific IgG, and to remove rheumatoid factors. This pre-treatment avoids false negative or false positive results. Microtiter wells as a solid phase are coated with Treponema pallidum antigen. Pre- treated patient specimens and ready-to-use controls are pipetted into these wells. During incubation, Treponema pallidum-specific antibodies of positive specimens and controls are bound to the immobilized antigens.After the washing step, that removes unbound sample and controls material HRP- conjugated anti-human IgM antibodies are then dispensed into the wells. During the second incubation, this anti-IgM conjugate binds specifically to IgM antibodies that forms enzyme-linked immune complexes. After a second washing step to remove unbound conjugate, the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and a blue color develops. The blue color turns to yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Treponema pallidum- specific IgM antibody in the patient’s serum specimen. An ELISA microtiter plate reader with an absorbance of 450 nm is used to read the kit.Specificity: 95%Sensitivity: 95%Kit Components:Microtiter Plate, 1x96wellsSample DIluent, 1x100mlIgG-RF-Sorbent, 1x6.5mlPositive Control, 1x2mlNegative Control, 1x2mlCut-off Control, 1x2mlEnzyme Conjugate (HRP), 1x20mlSubstrate Solution (TMB), 1x14mlStop Solution, 1x14mlWash Solution (20X), 1x30mlStorage and Stability:Store all components at 4°C. Stable for 6 months. Reconstituted standards are stable for two weeks stored at 4ºC. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Treponema pallidum IgM (Syphilis) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Treponema pallidum IgM (Syphilis) BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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