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细胞分析试剂盒
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中文名称

Tetanus Toxoid BioAssay ELISA Kit

英文名字
Tetanus Toxoid BioAssay ELISA Kit
供应商
USBiological
产品货号
167564
产品报价
¥1/96Tests
产品说明书
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背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Anti-tetanus toxoid antibodies are raised in response to vaccination with tetanus toxoid protein. A patient’s response to the immunization may be assessed, subsequently, by the serological determination of their anti-tetanus toxoid antibody levels using this quantitative enzyme immunoassay technique. Patients with recurrent infections should be investigated for immunodeficiency due to thymic abnormalities, and the consequent inability to respond to specific bacterial antigens (review1). Intended Use:Anti-Tetanus is intended for the in-vitro measurement of specific IgG antibodies against tetanus toxoid (T.Tox.) present in serum, in order to determine protective status. Sufficient materials are supplied to allow a maximum of 39 samples to be tested in duplicate or 87 in single, with a seven point calibration curve and two controls. The seven point calibration curve can be reduced to five points if measurement below 0.09 IU/ml is not required.Sensitivity: 0.0093 IU/mlRange: 0.01-7.0IU/mlSpecificity: 97.10%Test Principle:Microwells are pre-coated with tetanus toxoid antigen. The calibrators, controls and diluted patient samples are added to the wells and antibodies recognising the tetanus toxoid antigen bind during the first incubation. After washing the wells to remove all unbound proteins, purified peroxidase labelled rabbit anti- human IgG (y chain specific) conjugate is added. The conjugate binds to the captured human antibody and the excess unbound conjugate is removed by a further wash step. The bound conjugate is visualised with 3,3’,5,5’ tetramethylbenzidine (TMB) substrate which gives a blue reaction product, the intensity of which is proportional to the concentration of antibody in the sample. Phosphoric acid is added to each well to stop the reaction. This produces a yellow end point color, which is read at 450nm. Kit Components:1. Coated Wells, 12x8well strips2. Sample Diluent, 2x50ml3. IgG Calibrators, 7x1.2ml4. IgG High Control, 1x1.2ml5. IgG Low Control, 1x1.2ml6. IgG Conjugate, 1x12ml7. TMB Substrate, 1x14ml8. Stop Solution, 1x14mlStorage and Stability:Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对Tetanus Toxoid BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Tetanus Toxoid BioAssay ELISA Kit产品后将其置于4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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