- 中文名称
Neuron-Specific Enolase (NSE) BioAssay ELISA Kit
- 英文名字
- Neuron-Specific Enolase (NSE) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- 167562
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The glycolytic enzyme enolase (2-phosph-D-glycerate hydrolyase) exists as several dimeric isoenzymes (aa, ab, ay and yy) composed of three distinct subunits, a, b, and y. Three isoenzymes are found in human brain: aa, ay and yy. The ay and yy-enolase isoenzymes are also known as neuron-specific enolase (NSE) as these isoenzymes initially were detected in neurons and neuronendocrine cells. The NSE levels are low in health and benign subjects. Elevated levels are commonly found in patients with malignant tumors with neuronendocrine differentiation, especially small cell lung cancer and neuroblastoma. Lung cancer is one of the most spread cancer forms with incidences about 50~100 per 100,000 population. Approximately 20% of the lung cancer is small cell lung cancer. Patients with small cell lung cancer show various proportions of ay and yy isoenzyme. The determination of NSE should detect and y isoforms with the same sensitivity (1). The antibodies for this particular assay are specific for the y-subunit without cross reactivity with a or b subunits(1). NSE Kits are reported to be useful diagnostic marker for lung cancer (2), neuroblastoma (3), and melanoma (4), seminoma (S) and in injury of central nervous system (6). In addition to the above, NSE can be a valuable tool in following-up the effect of chemotherapy of small cell lung cancer, in prognostic evaluation of patients with small cell lung cancer.Intended use:The Neuron-Specific Enolase (NSE) enzyme linked immunosorbent assay (ELISA) provides quantitative measurement of human NSE in serum to aid in the clinical evaluation of patients suspected of having small cell lung cancer, and other related diseases.cer, and in differential diagnosis between cell lung cancer and non-small cell lung cancer.Test PrincipleThe NSE Quantitative Test Kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one monoclonal anti-γNSE antibody for solid phase (microtiter wells) immobilization and another monoclonal anti-γNSE antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The standards and test specimen (serum) are added to the antibody coated microtiter wells. During the incubation, specific NSE bound to anti-NSE antibody on the wells. Unbound NSE antigen is removed by washing the wells with buffer. Enzyme conjugate is then added to each well. After another incubation, unbound enzyme conjugate is washed off and the amount of bound peroxidase is proportional to the concentration of the NSE present in each sample. Upon addition of the substrate and chromogen, the intensity of blue color will develop in proportion to the concentration of NSE antigen in the samples.Detection Range: 0-200ng/mlSpecificity: 98.7%Sensitivity: 1.5ng/mlKit Components: Microtiter Plate, 1x96wellsSample DIluent, 1x12mlEnzyme Conjugate Reagent, 1x12mlStandard 0ng/ml, 1x1vialStandard 5ng/ml, 1x1vialStandard 15ng/ml, 1x1vialStandard 40ng/ml, 1x1vialStandard 100ng/ml, 1x1vialStandard 200ng/ml, 1x1vialEnzyme Conjugate Reagent, 1x12mlTMB Substrate, 1x12mlStop Solution, 1x12mlStorage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Neuron-Specific Enolase (NSE) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Neuron-Specific Enolase (NSE) BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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