- 中文名称
Insulin BioAssay ELISA Kit
- 英文名字
- Insulin BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- 167560
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Insulin BioAssay ELISA Kit is a solid phase enzyme-linked immunosorbant assay (ELISA). This test is designed for in vitro quantitative measurement of insulin in human serum and plasma.Principle of the Assay:Insulin is a solid phase enzyme-linked immunosorbant assay (ELISA). The wells are coated with monoclonal antibody with higher activity for insulin. When the samples, and controls are incubated in the wells with enzyme conjugate, which is another antibodies linked to horseradish peroxidase to form a sandwich complex bound tothe well. Unbound conjugate are then washed off with wash buffer. The amount of bounded peroxidase is proportional to the concentration of the insulin present in the sample. Upon addition of the substrate and chromogen, the intensity of color will develop in proportional to the concentration of insulin in the samples.Summary and Explanation of Test: Insulin is the principal hormone responsible for glucose metabolism. It is synthesized in the cells of the islets of Langerhans as the precuror, proinsulin, which is processed to form C-peptide and insulin and both are secreted in equimolar amounts into the portal circulation. The mature insulin molecule comprises two polypeptide chairs, the A chain (21 amino acids) and the B chain (30 amino acids), which are linked by two inter-chain disulphide bridges. There is, in addition, a single intra-chain disulphide bridge in the A chain. The sequence of insulin is highly conserved in mammalian species, and is homologous with the insulin-like growth factors IGF-I and IGF-II. Secretion of insulin is mainly controlled by plasma glucose concentration and the hormones have a number of important metabolic actions. Its principal function is to control the uptake and utilization of glucose in peripheral tissues via the glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by the hyperglycaemic hormones including glucagons, epinephrine(adrenaline), growth hormone and cortisol. Insulin concentrations are severely reduced in insulin-dependent diabetes (DDM) and some other conditions such as hypopituitarism. Insulin concentrations may be raised in non-insulin- dependant diabetes (NIDDM), obesity, insulinoma and some endocrine dysfunctions such as Cushing’s Syndrome and Acromegaly1, 2.The main clinical utility measurement is in the investigation of hypoglycaemia. Insulin assay have been used in the following applications:1. To assess the residual cell function, especially in newly diagnosed cases of IDDM. 2. As an aid to the discrimination between IDDM and NIDDM. 3. The diagnosis of insulinoma. 4. In the investigation of the pathophysiology of diabetes mellitus.Insulin assays are the essentials in various dynamic tests, such as oral of intravenous glucose tolerance tests (OGTT and IVGTT), to determine the insulin response of the pancreas and the degree of insulin resistance. In many applications, insulin measurements may be complicated by cross-reactivity with partially degraded insulin, proinsulin and split forms of proinsulin. Immune complexes of these molecules are essentially problematic in patients who have developed anti-insulin antibodies through animal insulin administration. The Insulin assay measures biologically active insulin with a high degree of specificity, using a pair of specific antibodies.Sensitivity:1.5uIU/mlKit Components:167560A: Microtiter Plate, 1x96 wells167560B: Enzyme Conjugate (HRP), 1x13ml167560C: Standard A (0uIU/mL), 1x2ml167560D: Standard B (5uIU/mL), 1x2ml167560E: Standard C (25uIU/mL), 1x2ml167560F: Standard D (50uIU/mL), 1x2ml167560G: Standard E (100uIU/mL), 1x2ml167560H: Standard F (200uIU/mL), 1x2ml167560J: TMB Solution A: 1x7ml167560J: TMB Solution B: 1x7ml167560L: Concentrated Wash Buffer (10X), 1x20ml167560M: Stop Solution, 1x8mlStorage and Stability:Store all components at 4°C. Stable for 6 months. Reconstituted standards are stable for two weeks stored at 4ºC. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Insulin BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Insulin BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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