- 中文名称
Free Beta Chorionic Gonadotropin (hCGbeta) BioAssay ECL ELISA Kit (Human)
- 英文名字
- Free Beta Chorionic Gonadotropin (hCGbeta) BioAssay ECL ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- 167552
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Human Chorionic Gonadotropin (hCG) is a glycoprotein hormone normally produced by placenta during pregnancy. The hormone is present in blood and urine around seven to thirteen days following implantation of the fertilized ovum. Structurally intact hCG molecules consist of two non-covalently linked polypeptide subunits, the alpha and beta chain subunits. Measurement of intact hCG and of the alpha subunit of hCG appears to give similar results in blood and urine but not the levels of beta subunit. In the normal second-trimester maternal sera, the level of intact hCG range from 20,000 mIU/ml to 50,000 mIU/ml. In contrast, the levels of either free ?- or free ?-hCG are on average one half of 1% of hCG levels. hCG and the free subunits appear not to be useful as serological markers for nontrophoblastic tumors; however, the absolute increase of ?-hCG level in choriocarcinoma patients clearly differentiates it from normal pregnancy.Recent studies showed a significant increase in the level of free ?-hCG subunit in trisomy 21 cases as compared with controls. Hence, it has been suggested that free ?-hCG subunit assay in a combination of maternal serum AFP could be effective in a screening protocol for trisomy 21.Intended Use:The free beta hCG-subunit quantitative assay is designed for in vitro quantitative measurement of human chorionic gonadotropin free beta-subunit in patient’s serum. (For professional use only).Principle of the Test:The free beta-hCG Quantitative Test Kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one anti-beta-hCG antibody for solid phase (microtiter wells) immobilization and another mouse monoclonal anti-beta-hCG antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test specimen (serum) is added to the beta-hCG antibody coated microtiterwells and incubated with the Zero Buffer. If beta-hCG is present in the specimen, it will combine with the antibody on the well. The well is then washed to remove any residual test specimen, and beta-hCG antibody labeled with horseradish peroxidase (conjugate) is added. The conjugate will bind immunologically to the beta-hCG on the well, resulting in the beta-hCG molecules being sandwiched between the solid phase and enzyme- linked antibodies. After an incubation at room temperature, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of 2N HCl, and the color is changed to yellow and measured spectrophotometrically at 450 nm. The concentration of beta-hCG is directly proportional to the color intensity of the test sample.Detection Range: 0-50U/mlSpecificity: 100%Sensitivity: 0.5U/mlKit Components:Microtiter Plate, 1x96wellsStandard 0U/ml, 1x1vialStandard 2.5U/ml, 1x1vialStandard 5U/ml, 1x1vialStandard 10U/ml, 1x1vialStandard 25U/ml, 1x1vialStandard 50U/ml, 1x1vialSample Diluent, 1x20mlEnzyme Conjugate Reagent, 1x18mlTMB Substrate, 1x12mlWash Buffer Concentrate (50X), 1x15mlStop Solution, 1x12mlStorage and Stability:Store all components at 4°C. Stable for 6 months. Reconstituted standards are stable for two weeks stored at 4ºC. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Free Beta Chorionic Gonadotropin (hCGbeta) BioAssay ECL ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Free Beta Chorionic Gonadotropin (hCGbeta) BioAssay ECL ELISA Kit (Human)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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