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细胞分析试剂盒
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中文名称

Carcinoembryonic Antigen (CEA, CD66e) BioAssay ELISA Kit Not for use in USA, for Export use only

英文名字
Carcinoembryonic Antigen (CEA, CD66e) BioAssay ELISA Kit Not for use in USA, for Export use only
供应商
USBiological
产品货号
167549
产品报价
¥1/96Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Carcinoembryonic antigen (CEA) is a cell-surface 200-kd glycoprotein. In 1969, it was reported that plasma CEA was elevated in 35 of 36 patients with adenocarcinoma of the colon and that CEA titers decreased after successful surgery. Normal levels were observed in all patients with other forms of cancer or benign diseases. Subsequent studies have not confirmed these initial findings, and it is now understood that elevated levels of CEA are found in many cancers. Increased levels of CEA are observed in more than 30% of patients with cancer of the lung, liver, pancreas, breast, colon, head or neck, bladder, cervix, and prostate. Elevated plasma levels are related to the stage and extent of the disease, the degree of differentiation of the tumor, and the site of metastasis. CEA is also found in normal tissue.Intended Use: The CEA enzyme immunoassay test kit is intended for the quantitative determination of CEA concentration in human serum.Principle of the Test:The CEA Quantitative Test Kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one monoclonal anti-CEA antibody for solid phase (microtiter wells) immobilization and another mouse monoclonal anti-CEA antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The standards and test specimen (serum) are added to the CEA antibody coated microtiter wells. Then CEA antibody labeled with horseradish peroxidase (conjugate) is added. If human CEA is present in the specimen, it will combine with the antibody on the well and the enzyme conjugate resulting in the CEA molecules being sandwiched between the solid phase and enzyme-linked antibodies. After 1 hour incubation at room temperature, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of 2N HCl. The color is changed to yellow and measured spectrophotometrically at 450 nm. The concentration of CEA is directly proportional to the color intensity of the test sample.Detection Range: 0-120ng/mlSpecificity: 98.7%Sensitivity: 1.0ng/mlKit Components:Microtiter Plate, 1x96wellsCEA Standard 0ng/ml, 2x500ulCEA Standard 3ng/ml, 2x500ulCEA Standard 12ng/ml, 2x500ulCEA Standard 30ng/ml, 2x500ulCEA Standard 60ng/ml, 2x500ulCEA Standard 120ng/ml, 2x500ulEnzyme Conjugate Reagent, 1x12 mlSubstrate TMB, 1x12mlStop Solution, 1x12mlWash Buffer Concentrate (50X), 1x15mlStorage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
产品特点
针对Carcinoembryonic Antigen (CEA, CD66e) BioAssay ELISA Kit Not for use in USA, for Export use only该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Carcinoembryonic Antigen (CEA, CD66e) BioAssay ELISA Kit Not for use in USA, for Export use only产品后将其置于4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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