- 中文名称
Brucella IgM BioAssay ELISA Kit
- 英文名字
- Brucella IgM BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- 167545
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Brucellosis is an infectious disease caused by small ellipsoid, gram-negative bacteria. There are four different germs: Br. abortus, Br. melitensis, Br. suis and Br. canis. People are infected by contact with infected animals or by eating meat or drinking unpasteurized milk from infected animals. As a rule, infected humans are not contagious. Brucellosis is most frequent in young and middle-aged individuals. Endangered persons are butchers, farmers, owners of pets, veterinaries and tourists in Southern countries. The appearance of brucellosis shows a prevalence during winter and spring.The incubation period is between one and three weeks, but may be as long as two months. Br. abortus and Br. melitensis may cause Bang’s Disease, or in rare cases Malta Fever. The first appears occasionally with a low pathogenicity for man. Typical symptoms for Bang’s Disease are periodically occurring fever, splenomegaly and swelling of lymph nodes. In some cases an inflammation of different joints and organs occurs. The Malta Fever is caused by the epidemic type of brucellosis, and infection almost always leads to a manifest illness. Some infections with Brucella can cause Brucella Hepatitis. It is possible that there is a link between an infection with Brucella and the outbreak of multiple sclerosis.During an antibiotic therapy or a chronic infection, the detection of Brucella spec. in blood, urine, cerebrospinal fluid, sputum or other body fluids could be negative. Serological methods like agglutination, complement fixation reaction, Brucella Coombs test and ELISA are good alternatives. The monitoring of antibodies can serve as a usual indication of the status of infection. During the first days, IgM is the only immunoglobulin that appears. As the disease progresses, IgM recedes quantitatively and IgG becomes predominant. In chronic brucellosis, IgG may be produced for an extended period.Intended Use:The Brucella IgM Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgM antibodies against Brucella in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service of United States Biological. This assay is intended for in-vitro diagnostic use only.Laboratory results can never be the only base of a medical report. The patient history and further tests have additionally to be taken into account.Principle of the Test:The Brucella IgM antibody test kit is based on the principle of the enzyme immunoassay (EIA). Brucella antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Brucella antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgM antibodies is directly proportional to the intensity of the color.Specificity: 100%Sensitivity: 100%Kit Components:167545A: Microtiter plate, 1x12 Brucella antigen coated microtiter strip167545B: Calibrator A (Negative Control), 1x2ml 167545C: Calibrator B (Cut-Off Standard), 1x2ml 167545D: Calibrator C (Weak Positive Control), 1x2ml 167545E: Calibrator D (Positive Control), 1x2ml 167545F: Enzyme Conjugate, 15ml167545G: Substrate, 1x15ml167545H: Stop Solution, 1x15ml167545K: Sample Diluent, 1x60ml 167545L: Wash Solution 10X (PBS + Tween 20), 1x60mlWash Buffer (10×), 1x60mlStorage and Stability:Store all components at 4°C. Stable for 6 months. Reconstituted standards are stable for two weeks stored at 4ºC. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Brucella IgM BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Brucella IgM BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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