- 中文名称
17-alpha hydroxy Progesterone BioAssay ELISA Kit Not for use in USA, for Export use only
- 英文名字
- 17-alpha hydroxy Progesterone BioAssay ELISA Kit Not for use in USA, for Export use only
- 供应商
- USBiological
- 产品货号
- 167543
- 产品报价
- ¥1/96Tests

- 产品说明书
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- 产品新闻

- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
- 产品描述
- 17-alpha hydroxy Progesterone BioAssay ELISA Kit is a C-21 steroid hormone produced in the adrenal gland and gonads, during the synthesis of glucocorticoids and sex steroids. It is derived from progesterone via 17-hydroxylase, a P450c17 enzyme, or from 17-hydroxypregnenolone via 3ß-hydroxysteroid dehydrogenase/?5-4 isomerase. 17a -OHP has no defined physiologic role except as a precursor molecule. Serum 17a -OHP levels are age-dependent, with peak levels observed during fetal life and the immediate postnatal period. During the first week of life, serum 17a -OHP levels fall ~50-fold as compared to cord blood values. A small transient increase occurs in male infants 30-60 days postnatally. Levels for both sexes remain at constant low levels during childhood, and then progressively increase during puberty reaching adult levels of ~100ng/dl (~3.03nmol/l). As with cortisol, serum 17a -OHP levels normally have an ACTH-dependent diurnal variation, with peak levels in the morning and a nadir at night.In addition, ovarian production of 17a -OHP increases during the luteal phase of the menstrual cycle. 17-hydroxyprogesterone is a natural progestin and in pregnancy increases in the third trimester primarily due to fetal adrenal production. Normal levels are 3-90ng/dl in children and in women, 15-70ng/dl prior to ovulation, and 35-290 ng/dl during the luteal phase. Measurements of levels of 17-hydroxyprogesterone are useful in the evaluation of patients with suspected congenital adrenal hyperplasia as the typical enzymes that are defective, namely 21-hydroxylase and 11ß-hydroxilase, lead to a build-up of 17OHP. In contrast, the rare patient with 17a-hydroxylase deficiency will have very low or undetectable levels of 17OHP. Elevated serum 17 a -OHP levels at baseline and/or after ACTH stimulation have also been reported in other forms of adrenal hyperplasia.Intended Use:Competitive immunoenzymatic colorimetric method for quantitative determination of 17α OH Progesterone concentration in serum and plasmaPrinciple of the Test:17alpha-OH Progesterone (antigen) in the sample competes with horseradish peroxidase 17alpha-OH Progesterone (enzyme-labelled antigen) for binding onto the limited number of anti-17alpha-OH Progesterone coated on the microplates (solid phase).After incubation, the bound/free separation is performed by a simple solid-phase washing. The enzyme substrate (H2O2) and theTMB-substrate (TMB) are added. After an appropriate time has elapsed for maximum colour development, the enzyme reaction is stopped and the absorbances are determined. 17alpha-OH Progesterone concentration in the sample is calculated based on a series by a set of standard. The colour intensity is inversely proportional to the 17alpha-OH Progesterone concentration in the sample.Detection Range: 0-19.2ng/mlSpecificity: 100%Sensitivity: 0.1ng/mlKit Components:Microtiter Plate, 1x96wellsTMB Substrate, 1x15mlStop Solution, 1x15mlStandard 0, 1x1mlStandard 1, 1x1mlStandard 2, 1x1mlStandard 3, 1x1mlStandard 4, 1x1mlStandard 5, 1x1mlControl, 1x1mlConjugate (HRP), 1x6mlStorage and Stability:Store all components at 4°C. Stable for 6 months. Reconstituted standards are stable for two weeks stored at 4ºC. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对17-alpha hydroxy Progesterone BioAssay ELISA Kit Not for use in USA, for Export use only该产品的特点优势,欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到17-alpha hydroxy Progesterone BioAssay ELISA Kit Not for use in USA, for Export use only产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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