- 中文名称
Anti-Myeloperoxidase Antibody (Anti-MPO) BioAssay ELISA Kit (Human)
- 英文名字
- Anti-Myeloperoxidase Antibody (Anti-MPO) BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- 152287
- 产品报价
- ￥1/48Test ￥1/96Tests
- 产品说明书
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- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Human Anti-Myeloperoxidase Antibody (Anti-MPO) ELISA Kit is an enzyme immunoassay for the in vitro quantitative measurement of Anti-MPO in human serum, plasma and other biological fluids.Detection Range:3.125-200ng/mlSensitivity:<1.12ng/mlPrecision:Intra-Assay CV: <10%Inter-Assay CV: <12%Test Principle:The microtiter plate has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells and the liquid in each well containing unbound material is aspirated after the required incubation period. HRP-conjugated secondary antibody is then added. After incubation the unbound conjugate is washed off. After TMB substrate solution is added, those wells that contain Anti-MPO will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-MPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components:*152287A: Microtiter Plate, 96 wells, Pre-coated, ready to use*152287B: Standard, 2x1vial 152287C: Standard Diluent, 1x20ml*152287D: Detection Reagent A, 1x120ul 152287E: Assay Diluent A, 1x12ml152287F: TMB Substrate, 1x9ml152287G: Stop Solution, 1x6ml152287H: Wash Buffer, 30X, 1x20mlStorage and Stability:Store *152287A, *152287B and *152287D at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Materials Required But Not Supplied:1. Microplate reader with 450 ± 10nm filter.2. Precision single or multi-channel pipettes and disposable tips.3. Eppendorf Tubes for diluting samples.4. Deionized or distilled water.5. Absorbent paper for blotting the microtiter plate.6. Container for Wash SolutionSample Preparation and Storage:Serum:Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000xg. Assay freshly prepared serum immediately or store samples in aliquots at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Plasma:Collect plasma using EDTA or heparin as anticoagulant. Centrifuge samples for 15 minutes at 1000xg at 2-8°C within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquots at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Other Biological Fluids:Centrifuge samples for 20 minutes at 1000×g. Remove particulates and assay immediately or store samples in aliquots at -20°C or -80°C. Avoid repeated freeze/thaw cycles.Note:1. Samples to be used within 5 days may be stored at 4°C, otherwise samples must be stored at -20°C (≤1 month) or -80°C (≤2 months) to avoid loss of bioactivity and contamination.2. Sample hemolysis will influence the results so hemolyzed specimens should not be used.3. When performing the assay, bring samples to room temperature.
- 产品特点
- 针对Anti-Myeloperoxidase Antibody (Anti-MPO) BioAssay ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Anti-Myeloperoxidase Antibody (Anti-MPO) BioAssay ELISA Kit (Human)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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