Rat VEGF ELISA Cytokine Development kit contains the key components required for the quantitative measurement of natural and/or recombinant Rat VEGF in a sandwich ELISA format. Using the ELISA protocol described below, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay Rat VEGF in approximately 1000 ELISA plate wells.Detection Range:32–2000pg/mlCross Reactivity:When tested at 50ng/ml the following antigens exhibited 100% cross reactivity: Human VEGF 121, VEGF 165 Murine VEGFWhen tested at 50ng/ml the following antigens did not exhibit significantcross reactivity: Human VEGF-BReconstitution and Storage:Capture Antibody: 50ug of antigen-affinity purified rabbit anti-Rat VEGF containing 2.5mg D-mannitol.Centrifuge vial prior to opening. Reconstitute in 0.5ml sterile water for a concentration of 100ug/ml. Detection Antibody: 50ug of biotinylated antigen-affinity purified rabbit anti-Rat VEGF containing 2.5mg D-mannitol.Centrifuge vial prior to opening. Reconstitute in 0.5ml sterile water for a concentration of 100ug/ml. Rat VEGF Standard: 1ug of recombinant Rat VEGF containing 2.2mg BSA, 11.0mg D-mannitol.Centrifuge vial prior to opening. Reconstitute in 1ml sterile water for a concentration of 1ug/ml. Note:The reconstituted components are stable for 2 weeks when stored at 2-8°C.Components that have been reconstituted and aliquoted can be stored at -20°C for up to 6 months. Avidin-HRP Conjugate: 60ul vial. Upon receipt, Avidin-HRP conjugate should be aliquoted into ten 6ul vials and stored at -20°C or lower. Aliquots stored frozen at below -20°C are stable for up to 2 years from date of receipt. Avoid more than one freeze-thaw cycle. Avidin should be used in conjunction with ABTS only.Buffer Kit Components NOT Included (order Catalog No 145859): ELISA microplates, 10x96 wellSealing FilmPBSWash BufferBlocking BufferDiluentABTS Liquid SubstrateExpiration Date:One year from date of receiptNote:Additional components can be purchased with our ELISA Buffer Kit: Cat.# 145859
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保存建议
建议收到VEGF Rat BioAssay ELISA Development Kit产品后将其置于4°C/-20°C保存。
