Rat GRO/KC ELISA Cytokine Development kit contains the key components required for the quantitative measurement of natural and/or recombinant Rat GRO/KC in a sandwich ELISA format. Using the ELISA protocol described below, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay Rat GRO/KC in approximately 1000 ELISA plate wells.Detection Range:16–1000pg/mlCross Reactivity:When tested at 50ng/ml the following antigens exhibited 100% cross reactivity: Murine KCWhen tested at 50ng/ml the following antigens exhibited less than 1% cross reactivity: Human GRO/MGSA, GRO beta, GRO y Murine MIP-2Rat GRO beta/MIP-2Reconstitution and Storage:Capture Antibody: 50ug of antigen-affinity purified rabbit anti-Rat GRO/KC containing 2.5mg D-mannitol.Centrifuge vial prior to opening. Reconstitute in 0.5ml sterile water for a concentration of 100ug/ml. Detection Antibody: 50ug of biotinylated antigen-affinity purified rabbit anti-Rat GRO/KC containing 2.5mg D-mannitol.Centrifuge vial prior to opening. Reconstitute in 0.5ml sterile water for a concentration of 100ug/ml. Rat GRO/KC Standard: 1ug of recombinant Rat GRO/KC containing 2.2mg BSA, 11.0mg D-mannitol.Centrifuge vial prior to opening. Reconstitute in 1ml sterile water for a concentration of 1ug/ml. Note:The reconstituted components are stable for 2 weeks when stored at 2-8°C.Components that have been reconstituted and aliquoted can be stored at -20°C for up to 6 months. Avidin-HRP Conjugate: 60ul vial. Upon receipt, Avidin-HRP conjugate should be aliquoted into ten 6ul vials and stored at -20°C or lower. Aliquots stored frozen at below -20°C are stable for up to 2 years from date of receipt. Avoid more than one freeze-thaw cycle. Avidin should be used in conjunction with ABTS only.Buffer Kit Components NOT Included (order Catalog No 145859): ELISA microplates, 10x96 wellSealing FilmPBSWash BufferBlocking BufferDiluentABTS Liquid SubstrateExpiration Date:One year from date of receiptNote:Additional components can be purchased with our ELISA Buffer Kit: Cat.# 145859
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保存建议
建议收到GRO/KC Rat BioAssay ELISA Development Kit产品后将其置于4°C/-20°C保存。
