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中文名称

MFG-E8, Human, BioAssay ELISA Kit (BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, SED1)

英文名字
MFG-E8, Human, BioAssay ELISA Kit (BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, SED1)
供应商
USBiological
产品货号
145755
产品报价
¥1/1Kit
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
MFG-E8, originally identified as a product of lactating mammary glands, is also secreted by activated macrophages and immature dendritic cells. MFG-E8 functions as an opsonin by binding phosphatidylserine on apoptotic cells and enhancing their phagocytosis.Sample Type:Cell culture supernates, serum, EDTA plasma, heparin plasma, saliva, urine and human milk.Intended Use:The Human MFG-E8 BioAssay is a 4.5 hour solid phase ELISA designed to measure MFG-E8 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human MFG-E8 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MFG-E8. Results obtained using natural human MFG-E8 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MFG-E8.Sensitivity:13.4pg/mlRange:~62.5-4000pg/ml (Serum, heparin plasma, saliva, cell culture supernates, human milk, EDTA plasma, urine)Specificity:Recognizes natural and recombinant human MFG-E8.Test Principle:This BioAssay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human MFG-E8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any human MFG-E8 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for human MFG-E8 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human MFG-E8 bound in the initial step. The color development is stopped and the intensity of the color is measured.Kit Components:Mfg-e8 Microplate, 1x96wellsMfg-e8 Standard, lyophilized, 1x40ngMfg-e8 Conjugate, 1x21mlAssay Diluent rD1-57, 1x11mlCalibrator Diluent rD5-20, 1x21mlWash Buffer Concentrate, 25X concentrated, 1x21mlColor Reagent A, 1x12mlColor Reagent B, 1x12mlStop Solution, 1x6mlPlate Sealers, 4x adhesive stripsStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对MFG-E8, Human, BioAssay ELISA Kit (BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, SED1)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到MFG-E8, Human, BioAssay ELISA Kit (BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, SED1)产品后将其置于4°C/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
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