- 中文名称
IGFBP-2, BioAssay ELISA Kit (Human) (Insulin Like Growth Factor Binding Protein 2)
- 英文名字
- IGFBP-2, BioAssay ELISA Kit (Human) (Insulin Like Growth Factor Binding Protein 2)
- 供应商
- USBiological
- 产品货号
- 145744
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The superfamily of secreted insulin-like growth factor (IGF) binding proteins includes high-affinity IGF binding proteins (IGFBP) and low-affinity binding proteins referred to as IGFBP-like or IGFBP- related proteins (IGFBPL, IGFBP-rP) (1). IGFBPs form complexes with IGF-I and -II, modulating their activity and bioavailability by modifying their binding to cell receptors, cell surfaces, and the extracellular matrix (1-5). In addition to direct effects on activity and binding of IGFs, IGFBPs actas carriers for circulating IGFs, prolonging their half-lives, and creating IGF reservoirs in tissues (1, 3, 4). IGFBP-2 and other superfamily members contain N-terminal IGF-binding domains with 12 conserved cysteines, and C-terminal thyroglobulin type 1 domains with 6 conserved cysteines (1). The C-terminal domain mediates heparin binding, which is enhanced in the presence of IGFs and, in the case of IGFBP-2, contains an RGD sequence that promotes integrin-mediated cell attachment (1, 3, 5-9). IGFBP-2 affinity and extracellular matrix binding enhancement is stronger for IGF-II than IGF-I (4, 9).Intended Use:For the quantitative determination of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2) concentrations in cell culture supernates, serum, plasma, and urine.Sensitivity:0.09ng/mlRange:~0.313-20ng/mlTest Principle:This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IGFBP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGFBP-2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IGFBP-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGFBP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.Kit Components:Microplate, 1x96wellsConjugate (HRP), 1x21mlStandard, 1x200ngAssay Diluent, 1x11mlCalibrator Diluent, 1x2vialsWash Buffer Concentrate, 1x21mlColor Reagent A, 1x12mlColor Reagent B, 1x12ml Stop Solution, 1x6mlPlate Sealers, 1x4 4 adhesive strips.Storage and Stability:Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对IGFBP-2, BioAssay ELISA Kit (Human) (Insulin Like Growth Factor Binding Protein 2)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到IGFBP-2, BioAssay ELISA Kit (Human) (Insulin Like Growth Factor Binding Protein 2)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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