- 中文名称
Tumor Necrosis Factor alpha (TNFa), Rat, BioAssay ELISA Kit
- 英文名字
- Tumor Necrosis Factor alpha (TNFa), Rat, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- 144064
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer.1 Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology.2 TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro.3 TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids.4 TNF-alpha has been mapped to human chromosome 6.2 The standard product used in this kit is recombinant rat TNFα, consisting of 157 amino acids with the molecular mass of 17kD.Principle of the Assay:The Tumor Necrosis Factor alpha (TNFa), BioAssay ELISA Kit (Rat) is based on standard sandwich enzyme-linked immunosorbent assay technology. A mouse monoclonal antibody specific for TNFa has been pre-coated onto 96-well plates. Standards (E. coli-expressed recombinant fragment corresponding to L80 thru L235 of rat TNFa) and test samples are added to the wells, incubated and then biotinylated detection goat polyclonal antibody specific for TNFa is added, followed by an incubation step and a washing step with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize the HRP-mediated reaction. TMB is catalyzed by HRP to produce a blue colored product that changes into yellow upon addition of acidic stop solution. The intensity of the yellow color is proportional to the amount of rat TNFa in the sample.This kit is intended for the quantitative determination of rat TNFa in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).Detection Range:15.6pg/ml-1000pg/mlSensitivity:< 1 pg/mlPrecision:Intra-assay: <10% (CV)Inter-Assay: <10% (CV)Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.Kit Components:144064A: Microtiter Plate, 1x96wells. Pre-coated with anti-rat TNFa antibody144064B: TNFa standard, 2x10ng144064C: Anti-rat TNFa (Biotin), 1x130ul (dilute 1:100)143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilute 1:100)143697: Sample Diluent Buffer, 1x30ml143698: Antibody Diluent Buffer, 1x12ml143699: ABC Diluent Buffer, 1x12ml143700: TMB Color Developing Agent, 1x10ml143701: TMB Stop Solution, 2N H2SO4, 1x10mlStorage and Stability: Unopened kit is stable at 4°C for 6 months.Assay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 min. Do not wash. 2. Add biotinylated antibody and incubate the plate at 37°C for 60 min. Wash plate 3 times with 0.01M TBS or PBS.3. Add ABC working solution and incubate the plate at 37°C for 30min. Wash plate 5 times with 0.01M TBS or PBS.4. Add TMB color developing agent and incubate the plate at 37°C in dark for 20-25 min.5. Add Stop solution and read absorbance at 450nm.
- 产品特点
- 针对Tumor Necrosis Factor alpha (TNFa), Rat, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Tumor Necrosis Factor alpha (TNFa), Rat, BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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