- 中文名称
Leptin BioAssay ELISA Kit, Human
- 英文名字
- Leptin BioAssay ELISA Kit, Human
- 供应商
- USBiological
- 产品货号
- 143948
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue.1 Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively.2 Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart.3 Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling.4 Leptin is a powerful inhibitor of bone formation in vivo.5 Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q.3 The standard product used in this kit is recombinant human Leptin.Principle of the Assay:This Human Leptin ELISA Kit is a sandwich enzyme-linked immunosorbent assay for the in-vitro quantitative determination of leptin in human plasma, serum and cell culture supernatants. A mouse monoclonal antibody specific for human Leptin has been pre-coated onto the wells of the strip plate. Standards (recombinant human Leptin from expressed in E.coli, V22-C167) and test samples are added to the wells and biotinylated polyclonal goat detection antibody specific for human Leptin is subsequently added, followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB substrate is used to visualize the HRP-mediated enzymatic reaction. TMB, in a reaction catalyzed by HRP, produces a blue colored product that changes to yellow upon addition of an acidic stop solution. The intensity of the yellow color is proportional to the concentration of human Leptin in the sample.Assay Range:62.5pg/ml-4000pg/mlSensitivity:< 10 pg/mlCrossreactivity:There is no detectable cross-reactivity with other relevant proteins.Uniprot ID:A4D0Y8Entrez Gene ID:3952Kit Components:143948A: Microtiter Plate, 1x96 wells (12 x 8 strips). Pre-coated with anti-human Leptin antibody.143948B: Leptin Standard, 2x10ng. Lyophilized recombinant human Leptin.143948C: Biotinylated anti-Leptin Antibody, 1x130ul143948D: ABC Complex (Avidin-Biotin-Peroxidase Complex), 1x130ul143948E: Sample Diluent Buffer, 1x30ml143948F: Antibody Diluent Buffer, 1x12ml143948G: ABC Diluent Buffer, 1x12ml143948H: TMB Substrate, 1x10ml143948I: TMB Stop Solution, 1x10mlStorage and Stability:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.Summary of the Assay:1. Add samples and standards and incubate the plate at 37°C for 90 min. Do not wash.2. Add biotinylated antibody and incubate the plate at 37°C for 60 min. Wash plate 3 times with 0.01M TBS.3. Add ABC working solution and incubate the plate at 37°C for 30min. Wash plate 5 times with 0.01M TBS.4. Add TMB color developing agent and incubate the plate at 37°C in dark for 25-30 min.5. Add TMB stop solution and read.
- 产品特点
- 针对Leptin BioAssay ELISA Kit, Human该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Leptin BioAssay ELISA Kit, Human产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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