- 中文名称
Laminin BioAssay ELISA Kit, Human
- 英文名字
- Laminin BioAssay ELISA Kit, Human
- 供应商
- USBiological
- 产品货号
- 143945
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Sandwich ELISA quantitative detection kit for Human Laminin in cell culture supernates, serum and plasma (heparin, EDTA).Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3.3 The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31. Assay Range:156pg/ml-10,000pg/mlSensitivity:< 10 pg/mlCrossreactivity:There is no detectable cross-reactivity with other relevant proteins.Uniprot ID:B0YJ33Entrez Gene ID:3909Principle of the Assay:The Human Laminin ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A rabbit polyclonal antibody specific for Laminin has been pre-coated onto 96-well plates. Standards (from human fibroblasts) and test samples are added to the wells, incubated and then biotinylated detection polyclonal antibody from rabbit specific for Laminin is added, followed by an incubation step and a washing step with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue colored product that changes into yellow upon addition of acidic stop solution. The intensity of the yellow color is proportional to the amount of Laminin in the sample.Kit Components:143945A: Microtiter Plate, 1x96wells. Pre-coated with anti-human Laminin antibody143945B: Laminin standard, 2x10ng143945B: Anti-human Laminin (Biotin), 1x130ul (1:100 dilution)143696C: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution)143697: Sample Diluent Buffer, 1x30ml143698: Antibody Diluent Buffer, 1x12ml143699: ABC Diluent Buffer, 1x12ml143700: TMB Color Developing Agent, 1x10ml143701: TMB Stop Solution, 2N H2SO4, 1x10mlStorage and Stability: Unopened kit is stable at 4°C for 6 months.Material Required But Not Provided:1. Microplate reader in standard size2. Automated plate washer3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended when a large number of samples are to be assayed 4. Clean tubes and Eppendorf tubes5. Washing buffer (neutral PBS or TBS)Assay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 min. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 min. Wash plate 3 times with 0.01M TBS or PBS.3. Add AB Cworking solution and incubate the plate at 37°C for 30min. Wash plate 5times with 0.01M TBS or PBS.4. Add TMB color developing agent and incubate the plate at 37°C in dark for 15-20 min.5. Add Stop solution and read absorbance at 450nm.
- 产品特点
- 针对Laminin BioAssay ELISA Kit, Human该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Laminin BioAssay ELISA Kit, Human产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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