- 中文名称
Interleukin-16 (IL-16) BioAssay ELISA Kit (Human)
- 英文名字
- Interleukin-16 (IL-16) BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- 143898
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Sandwich ELISA quantitative detection kit for Human IL-16 in cell culture supernates, serum and plasma (heparin, EDTA).Interleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. By Southern blot analysis and PCR using a human/rodent somatic cell hybrid mapping panel, The human IL16 is encoded by a single-copy gene on chromosome 15. Using a combination of STS-content mapping, radiation-hybrid mapping, and genetic mapping, it was refined the assignment to 15q26.1. The mouse Il16 gene was mapped to chromosome 7 in a region showing homology of synteny to human 15q26.1. IL-16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.Uniprot ID:Q14005Entrez Gene ID:3603Intended Use:This kit is intended for the quantitative determination of human IL-16 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).Principle of the Assay:The Interleukin-16 (IL-16) BioAssay ELISA Kit (Human) is based on standard sandwich enzyme-linked immunosorbent assay technology. A mouse monoclonal antibody specific for IL-16 has been pre-coated onto 96-well plates. Standards (E. coli-expressed recombinant fragment corresponding to P2 thru S130 of human IL-16) and test samples are added to the wells, incubated and then biotinylated detection goat polyclonal antibody specific for IL-16 is added, followed by an incubation step and a washing step with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize the HRP-mediated reaction. TMB is catalyzed by HRP to produce a blue colored product that changes into yellow upon addition of acidic stop solution. The intensity of the yellow color is proportional to the amount of human IL-16 in the sample.Detection Range:31.2pg/ml - 2000pg/mlSensitivity:<10 pg/mlPrecision: Intra-Assay CV: <10%Inter-Assay CV: <10%Cross-reactivity:There is no detectable cross-reactivity with other relevant proteins.Kit Components:143898A: Microtiter Plate, 1x96wells. Pre-coated with anti-human IL-16 antibody143898B:IL-16 standard, 2x10ng143898C: Anti-human IL-16 (Biotin), 1x130ul (dilute 1:100)143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilute 1:100)143697: Sample Diluent Buffer, 1x30ml143698: Antibody Diluent Buffer, 1x12ml143699: ABC Diluent Buffer, 1x12ml143700: TMB Color Developing Agent, 1x10ml143701: TMB Stop Solution, 2N H2SO4, 1x10ml143702: Wash Buffer, 1x1 packet. PBS powder for preparation of 1L buffer.Storage and Stability: Unopened kit is stable at 4°C for 6 months.Assay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 min. Do not wash. 2. Add biotinylated antibody and incubate the plate at 37°C for 60 min. Wash plate 3 times with wash buffer.3. Add ABC working solution and incubate the plate at 37°C for 30min. Wash plate 5 times with wash buffer.4. Add TMB color developing agent and incubate the plate at 37°C in dark for 20-25 min.5. Add Stop solution and read absorbance at 450nm.
- 产品特点
- 针对Interleukin-16 (IL-16) BioAssay ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interleukin-16 (IL-16) BioAssay ELISA Kit (Human)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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