- 中文名称
Interleukin-1 beta, Human, BioAssay ELISA Kit (IL-1 beta, Catabolin, IL-1B, IL1F2, IL1-beta)
- 英文名字
- Interleukin-1 beta, Human, BioAssay ELISA Kit (IL-1 beta, Catabolin, IL-1B, IL1F2, IL1-beta)
- 供应商
- USBiological
- 产品货号
- 143885
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Sandwich ELISA quantitative detection kit for Human IL-1 beta in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Interleukin-1b ( IL-1b) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1b, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1b and, to a lesser extent, IL-1a are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1b and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1b, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.Assay Range:3.9-250pg/mlSensitivity:<0.15 pg/mlSpecificity:Recognizes native and recombinant human IL-1b.Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.Uniprot ID:P01584Entrez Gene ID:3553Kit Components:143885A: Microtiter Strip, 1x96-well Pre-coated with anti-IL-1 beta, Human 143885B: Standard, 1000pg/ml, 2x1vial Lyophilized recombinant IL-1 beta, Human 143885C: Pab (Biotin) GtxHu, 1x130ulUSB Cat NoKit Component143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution)143697: Sample diluent buffer, 1x30ml143698: Antibody diluent buffer, 1x12ml143699: ABC diluent buffer, 1x12ml143700: TMB color developing agent, 1x10ml143701: TMB stop solution, 2N H2SO4, 1x10mlStorage and Stability:Store *143885B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Summary1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS. 3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS. 4. Add TMB color developing agent and incubate thutese plate at 37°C in dark for 25-30 min. 5. Add TMB stop solution and read.
- 产品特点
- 针对Interleukin-1 beta, Human, BioAssay ELISA Kit (IL-1 beta, Catabolin, IL-1B, IL1F2, IL1-beta)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interleukin-1 beta, Human, BioAssay ELISA Kit (IL-1 beta, Catabolin, IL-1B, IL1F2, IL1-beta)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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