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中文名称

Fibroblast Growth Factor 21 (FGF21) BioAssay ELISA Kit, Human

英文名字
Fibroblast Growth Factor 21 (FGF21) BioAssay ELISA Kit, Human
供应商
USBiological
产品货号
143831
产品报价
¥1/96Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.Uniprot ID:Q9NSA1Entrez Gene ID:26291Intended Use:Fibroblast Growth Factor 21 (FGF21) BioAssay ELISA Kit, Human is a sandwich ELISA for quantitative detection of human FGF21 in cell culture supernates, serum and plasma (heparin, EDTA).Range:31.2-2000pg/mlSensitivity:<10pg/mlSpecificity:Natural and recombinant human FGF21Crossreactivity:No detectable crossreactivity with other relevant proteinsKit Components:143831A: Microtiter Strips, 1x96 wells*143831B: FGF21 Standard, 2x1 vial143831C: Anti-human FGF21 (Biotin), 1x130ulUSB Cat NoKit Component143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution)143697: Sample Diluent Buffer, 1x30ml143698: Antibody Diluent Buffer, 1x12ml143699: ABC Diluent Buffer, 1x12ml143700: TMB Color Developing Agent, 1x10ml143701: TMB Stop Solution, 2N H2SO4, 1x10mlAssay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS.3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS.4. Add TMB color developing agent and incubate the plate at 37°C in dark for 25-30 minutes.5. Add TMB stop solution and read.Storage and Stability:Store *143831B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对Fibroblast Growth Factor 21 (FGF21) BioAssay ELISA Kit, Human该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Fibroblast Growth Factor 21 (FGF21) BioAssay ELISA Kit, Human产品后将其置于4°C/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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