- 中文名称
FASL BioAssay ELISA Kit, Human
- 英文名字
- FASL BioAssay ELISA Kit, Human
- 供应商
- USBiological
- 产品货号
- 143829
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.Intended Use:For quantitative detection of human FASL in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Range:15.6-1000pg/mlSensitivity:< 2pg/mlSpecificity:Natural and recombinant human FASLCrossreactivity:No detectable crossreactivity with other relevant proteinsKit Components:143829A: Microtiter Strip, 1x96 wells143829B: FASL Standard, 2x10ng143829C: Anti-human FASL (Biotin), 130ulUSB Cat NoKit Component143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution)143697: Sample diluent buffer, 1x30ml143698: Antibody diluent buffer, 1x12ml143699: ABC diluent buffer, 1x12ml143700: TMB color developing agent, 1x10ml143701: TMB stop solution, 2N H2SO4, 1x10mlStorage and Stability:Store *143829B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Add 100ul of samples and standards and incubate the plate at 37°C for 90 min. Do not wash.2. Add 100ul biotinylated antibody and incubate the plate at 37°C for 60 min. Wash plate 3 times with 0.01M TBS or PBS.3. Add ABC working solution and incubate the plate at 37°C for 30min. Wash plate 5 times with 0.01M TBS or PBS.4. Add TMB color developing agent and incubate the plate at 37°C in dark for 20-25 min.5. Add 100ul TMB stop solution and read.
- 产品特点
- 针对FASL BioAssay ELISA Kit, Human该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到FASL BioAssay ELISA Kit, Human产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
