- 中文名称
Complement Component 5a (C5a) BioAssay ELISA Kit, Human
- 英文名字
- Complement Component 5a (C5a) BioAssay ELISA Kit, Human
- 供应商
- USBiological
- 产品货号
- 143780
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.Principle of the Assay:The Complement C5a (C5a), BioAssay ELISA Kit (Human) is based on standard sandwich enzyme-linked immunosorbent assay technology. A mouse monoclonal antibody specific for C5a has been pre-coated onto 96-well plates. Standards (E. coli-expressed recombinant fragment corresponding to T678 thru R751 of human C5a) and test samples are added to the wells, incubated and then biotinylated detection goat polyclonal antibody specific for C5a is added, followed by an incubation step and a washing step with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize the HRP-mediated reaction. TMB is catalyzed by HRP to produce a blue colored product that changes into yellow upon addition of acidic stop solution. The intensity of the yellow color is proportional to the amount of human C5a in the sample.This kit is intended for the quantitative determination of human C5a in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).Detection Range:31.2pg/ml-2000pg/mlSensitivity:<10 pg/mlPrecision:Intra-assay: <10% (CV)Inter-Assay: <10% (CV)Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.Kit Components:143780A: Microtiter Plate, 1x96wells. Pre-coated with anti-human C5a antibody143780B:C5a standard, 2x10ng143780C: Anti-human C5a (Biotin), 1x130ul (dilute 1:100)143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilute 1:100)143697: Sample Diluent Buffer, 1x30ml143698: Antibody Diluent Buffer, 1x12ml143699: ABC Diluent Buffer, 1x12ml143700: TMB Color Developing Agent, 1x10ml143701: TMB Stop Solution, 2N H2SO4, 1x10mlStorage and Stability: Unopened kit is stable at 4°C for 6 months.Assay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 min. Do not wash. 2. Add biotinylated antibody and incubate the plate at 37°C for 60 min. Wash plate 3 times with 0.01M TBS or PBS.3. Add ABC working solution and incubate the plate at 37°C for 30min. Wash plate 5 times with 0.01M TBS or PBS.4. Add TMB color developing agent and incubate the plate at 37°C in dark for 20-25 min.5. Add Stop solution and read absorbance at 450nm.
- 产品特点
- 针对Complement Component 5a (C5a) BioAssay ELISA Kit, Human该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Complement Component 5a (C5a) BioAssay ELISA Kit, Human产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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