- 中文名称
GST Cleavage Capture Kit
- 英文名字
- GST Cleavage Capture Kit
- 供应商
- USBiological
- 产品货号
- 137401
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The GST Cleavage Capture Kit allows easy release of free recombinant protein from their GST-tag when a Factor Xa recognition sequence is present.While GST-fusion proteins allow convenient affinity purification of many proteins of interest, the GST-tag may become inconvenient in various downstream applications. This GST Cleavage Capture Kit is designed for efficient removal of such GST-tags in fusion proteins where the amino acid sequence Ile-Glu(or Asp)-Gly-Arg-//-X is present in between the protein of interest and GST. This sequence is efficiently cleaved by the protease Factor Xa, and the two cleavage products (GST and protein of interest) can be easily separated. This cleavage reaction is usually completed within a few hours at temperatures between 4°C to 37°C, and the free GST-tag is removed by GSH-gel treatment.Kit Components:Factor Xa stock solution:10ug in 10ul Tris Buffer solution (20mM, ph 7.4) activity: 20,000 IU/mgPlease note that dilutions to a lower concentration than 1ug/10ul may result in loss of protein due to adsorption.Inhibitors:Phenylmethylsulfonyl fluoride (PMSF) 20ul 100x stockDithiothreitol (DTT) 20ul 100x stockChloromethylketone in NaClBoc-DPhe-Arg(Z2)-CH2-Cl dissolve in 25ul Cleavage BufferCL-Glutathione ChroMatrixTM 2x1mlWash Buffer (A) 50ml, contains 10mM mercaptoethanolWash Buffer (B) 10ml, contains 10mM mercaptoethanolElution Buffer (C) 25ml, contains 5mM DTTCleavage Buffer (D) 20ml, contains 1mM DTTElution Buffer (E) 5ml, contains 5mM GSHMaterials Required But Not Supplied:micropipettesequipment for GSH affinity chromatographycentrifugeSDS-PAGE and staining solutionsStorage & Stability:Quality guaranteed for 12 months Store at 4°C, keep Factor Xa at -80°C
- 产品特点
- 针对GST Cleavage Capture Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到GST Cleavage Capture Kit产品后将其置于4°C/-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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