- 中文名称
Sirt3, Fluorogenic Assay Kit, BioAssay (Sirtuin 3, SIR2L3)
- 英文名字
- Sirt3, Fluorogenic Assay Kit, BioAssay (Sirtuin 3, SIR2L3)
- 供应商
- USBiological
- 产品货号
- 137371
- 产品报价
- ￥1/32Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Fluorogenic SIRT3 Assay Kit is a complete assay system designed to measure Sirtuin 3 (SIRT3) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary. The kit includes purified SIRT3 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT3 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT3 activity level. First, the HDAC fluorometric substrate (HDAC substrate 1), containing an acetylated lysine side chain, is incubated with purified SIRT3 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.Intended Use:Designed to study enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.Kit Components:SIRT3 recombinant human enzyme: 1x70ugFluorogenic HDAC substrate 1: 1x50ul @5mMNicotinamide Adenine Dinucleotide (NAD+): 1x50ul @50mMNicotinamide: 1x500ul @10mM2x SIRT Developer (contains 2mM Nicotinamide): 1x6mlSIRT assay buffer: 1x10mlBlack, low binding NUNC black microtiter plate: 1 plateStorage and Stability:Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Sirt3, Fluorogenic Assay Kit, BioAssay (Sirtuin 3, SIR2L3)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Sirt3, Fluorogenic Assay Kit, BioAssay (Sirtuin 3, SIR2L3)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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