- 中文名称
Sirt1, Fluorogenic Assay Kit, BioAssay (NAD-dependent Deacetylase Sirtuin-1, hSIRT1, SIR2-like Protein 1, hSIR2, SIR2L1)
- 英文名字
- Sirt1, Fluorogenic Assay Kit, BioAssay (NAD-dependent Deacetylase Sirtuin-1, hSIRT1, SIR2-like Protein 1, hSIR2, SIR2L1)
- 供应商
- USBiological
- 产品货号
- 137369
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Fluorogenic SIRT1 Assay Kit is a complete assay system designed to measure Sirtuin 1 (SIRT1) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT1 (Sir2) activity measurements. In addition, the kit includes purified SIRT1 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT1 activity level. First, the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated lysine side chain, is incubated with a sample containing SIRT1 activity (e.g. HeLa nuclear extract or purified SIRT1 enzyme). The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.Intended Use:Designed to measure Sirtuin 1 (SIRT1) activity for screening and profiling applications.Test Principle:The Fluorogenic SIRT1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT1 activity level. First, the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated lysine side chain, is incubated with a sample containing SIRT1 activity (e.g. HeLa nuclear extract or purified SIRT1 enzyme). The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.Kit Components:SIRT1 recombinant human enzyme: 1x50ug (0.06mg/ml)Fluorogenic SIRT substrate 1: 1x50ul @5mMNicotinamide Adenine Dinucleotide (NAD+): 1x50ul @50mMNicotinamide: 1x500ul @10mM2x SIRT Developer (contains 2 mM Nicotinamide): 1x6mlTrichostatin A (200uM) 1x100ulSIRT assay buffer: 1x10mlBlack, low binding NUNC black microtiter plate: 1 plateStorage and Stability:Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Sirt1, Fluorogenic Assay Kit, BioAssay (NAD-dependent Deacetylase Sirtuin-1, hSIRT1, SIR2-like Protein 1, hSIR2, SIR2L1)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Sirt1, Fluorogenic Assay Kit, BioAssay (NAD-dependent Deacetylase Sirtuin-1, hSIRT1, SIR2-like Protein 1, hSIR2, SIR2L1)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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