- 中文名称
PDE4D3 TR-FRET Assay Kit, BioAssay
- 英文名字
- PDE4D3 TR-FRET Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- 137344
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4D is a regulator of airway smooth-muscle contractility, and has been identified as a potential risk predictor for ischemic stroke. Additionally, PDE4D has been associated with asthma pathophysiology and bone formation. The PDE4D gene encodes at least 9 different isoforms.Intended Use:The TR-FRET PDE4D3 Assay Kit is designed for identification of inhibitors of PDE4D3 using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology. The assay is based on the binding of phosphate groups from FAM-labeled nucleotide monophosphates generated by PDE4D3 and from terbium donors to the nanoparticle binding agent. The binding will result in energy transfer from terbium to FAM and the fluorescent intensity change can be measured using fluorescence reader. Test Principle:The TR-FRET PDE4D3 Assay Kit comes in a convenient 96-well format, with purified PDE4D3 enzyme, fluorescently labeled PDE4D3 substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. Using this kit, only two simple steps on a microtiter plate are required for the PDE4D3 activity assay. First, the fluorescent-labeled cAMP is incubated with a sample containing PDE4D3 for 1 hour. Second, a binding agent and a terbium donor are added to the reaction mix and incubated for 1 hour. Then, fluorescence intensity can be measured using a fluorescence reader.Kit Components:NUNC microtiter plate, 1x96wellsRecombinant enzyme, 1x1ugFAM-Cyclic-3’,5’-AMP 20uM, 1x50ulPDE assay buffer, 1x25mlTb Donor, 1x30ulBinding agent, 1x200ulBinding Buffer A, 1x20mlBinding Buffer B, 1x20mlMaterials or Instruments Required But Not Supplied:Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)Applications:Suitable for screening small molecular inhibitors for drug discovery and HTS applications.Storage and Stability:Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对PDE4D3 TR-FRET Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到PDE4D3 TR-FRET Assay Kit, BioAssay产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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