- 中文名称
Phosphodiesterase 4B2 (PDE4B2), BioAssay Kit
- 英文名字
- Phosphodiesterase 4B2 (PDE4B2), BioAssay Kit
- 供应商
- USBiological
- 产品货号
- 137341
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4 selective inhibitors are currently in clinical trials for the treatment of diseases related to inflammatory disorders. Increased expression of PDE4B2 was observed in the near-term myometrium. PDE4B2 can be induced by its own substrate, under the control of one of the major utero-contractile agonist, PGE2. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations
and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.The PDE4B2 Assay Kit is designed for identification of PDE4B2 inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4B2 to the binding agent.Test Priniciple:The PDE4B2 inhibitor screening assay kit comes in a convenient 96-well format, with purified PDE4B2 enzyme, fluorescently labeled PDE3 substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE4B2 Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4B2 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4B2 for 1 hour. Second, binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.Kit Components:137341A: Microtiter Plate, 1x96 wells*137341B: PDE4B2 Recombinant Enzyme, 1x5ug*137341C: FAM-Cyclic-3’,5’-AMP 20uM, 1x50ul*137341D: PDE Assay Buffer, 1x25ml137341E: Binding Agent, 1x100ul137341F: Binding Agent Diluent, 1x10mlStorage and Stability:Store *137341D at -20ºC. Store *137341B and *137341C at -70ºC. Store all other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Phosphodiesterase 4B2 (PDE4B2), BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Phosphodiesterase 4B2 (PDE4B2), BioAssay Kit产品后将其置于4°C/-20°C/-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
