- 中文名称
PCR Cloning BioGenomics Kit
- 英文名字
- PCR Cloning BioGenomics Kit
- 供应商
- USBiological
- 产品货号
- 137318
- 产品报价
- ￥1/10Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Description:The PCR Cloning Kit is a simple and highly efficient method to insert any gene or DNA fragment into a vector, without time-consuming ligation steps.• No ligation steps required• Multiple PCR inserts can be added in a single step• Efficiencies of > 90% are typical.• Go from PCR product to transforming cells in under an hour!• Suitable for fixing or adding point mutations, insertions or deletions.Test Principle:A primer set homologous to the gene of interest and the vector is used to amplify a PCR product. This purified PCR product is incubated with restriction enzyme-digested vector and Reagent A of the PCR Cloning BioAssay Kit. Following a brief incubation with Reagent B, the annealed vector is ready for transformation.Primer Design:Proper primer design is critical for successful PCR and transformation steps. Primers for the gene of interest should contain the restriction site as well as at least 10 nucleotides of sequence homologous to the vector sequence. Using two different restriction enzymes will allow directional cloning of the insert. Note: Once purified, the digested vector can be used with multiple inserts if the primers for insert amplification are designed correctly.Kit Components:137318A: Reagent A, 1x1vial137318B: Reagent B, 1x1vial137318C: Buffer A, 1x1vial137318D: BSA, 10X (0.1% w/v), 1x1vial137318E: Distilled Water (dH2O), 1x1vialStorage and Stability:Store all components at -20°C. Stable for 6 months after receipt. Aliquot into working volumes after first thawing of components to avoid multiple freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对PCR Cloning BioGenomics Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到PCR Cloning BioGenomics Kit产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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