- 中文名称
LEF1 Reporter, BioAssay Kit (Lymphoid Enhancer-binding Factor 1, LEF-1, T Cell-specific Transcription Factor 1-alpha, TCF1-alpha)
- 英文名字
- LEF1 Reporter, BioAssay Kit (Lymphoid Enhancer-binding Factor 1, LEF-1, T Cell-specific Transcription Factor 1-alpha, TCF1-alpha)
- 供应商
- USBiological
- 产品货号
- 137307
- 产品报价
- ￥1/500Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Wnt / b-catenin signaling pathway controls a large and diverse set of cell fate decisions in embryonic development, adult organ maintenance and disease. Wnt proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to stabilization and nuclear translocation of b-catenin. b-catenin then binds to TCF/LEF transcription factors in the nucleus, leading to transcription and expression of Wnt-responsive genes.Intended Use:Designed for monitoring the activity of Wnt / b-catenin signaling pathway in the cultured cells.Test Principle:The kit contains transfection- ready TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non- inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.Kit Components:Reporter (Component A): TCF/LEF luciferase reporter vector + constitutively expressing Renilla luciferase vector. 1x500ul (60ng DNA/ul)Negative Contol Reporter (Component B): Non-inducible luciferase vector + constitutively expressing Renilla luciferase vector. 1x500ul (60ng DNA/ul)Storage and Stability:Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对LEF1 Reporter, BioAssay Kit (Lymphoid Enhancer-binding Factor 1, LEF-1, T Cell-specific Transcription Factor 1-alpha, TCF1-alpha)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到LEF1 Reporter, BioAssay Kit (Lymphoid Enhancer-binding Factor 1, LEF-1, T Cell-specific Transcription Factor 1-alpha, TCF1-alpha)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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