- 中文名称
GCN5, Fluorogenic Assay Kit, BioAssay (KAT2A, Histone Acetyltransferase KAT2A, General Control of Amino Acid Synthesis Protein 5-like 2, Histone Acetyltransferase GCN5, HsGCN5, Lysine Acetyltransferas
- 英文名字
- GCN5, Fluorogenic Assay Kit, BioAssay (KAT2A, Histone Acetyltransferase KAT2A, General Control of Amino Acid Synthesis Protein 5-like 2, Histone Acetyltransferase GCN5, HsGCN5, Lysine Acetyltransferase 2A, STAF97, GCN5L2, HGCN5)
- 供应商
- USBiological
- 产品货号
- 137273
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Fluorogenic GCN5 Assay Kit is a homogeneous, fluorogenic assay designed to screen for inhibitors of GCN5 or to measure histone acetyltransferase (HAT) activity using cell extracts or purified enzymes. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HAT activity measurements. No time- consuming washing steps are required. In addition, the kit includes purified GCN5 (also known as KAT2A) for use as a positive control. The Fluorogenic GCN5 Assay Kit is based on the transfer of an acetyl group from acetyl CoA to a peptide substrate. After incubation with acetyl CoA and the substrate, the GCN5 enzyme generates acetylated H3 peptide and CoASH. The thiol groups of CoASH can be detected with fluorogenic reagent at excitation = 360nm and emission = 460nm.Intended Use:Designed to screen for inhibitors of GCN5 or to measure histone acetyltransferase (HAT) activity using cell extracts or purified enzymes.Test Principle:The Fluorogenic GCN5 Assay Kit is based on the transfer of an acetyl group from acetyl CoA to a peptide substrate. After incubation with acetyl CoA and the substrate, the GCN5 enzyme generates acetylated H3 peptide and CoASH. The thiol groups of CoASH can be detected with fluorogenic reagent at excitation = 360nm and emission = 460nm.Kit Components:GCN5 recombinant human enzyme: 1x25gAcetyl CoA (1mM): 1x100ulHAT assay buffer (10X): 1x5mlH3 peptide: 1x500ulFluorescence Developer: 1x250ulDeveloper Solution: 1x10mlHAT Stop Solution: 1x5mlBlack, low binding NUNC black microtiter plate: 1 plateStorage and Stability:Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对GCN5, Fluorogenic Assay Kit, BioAssay (KAT2A, Histone Acetyltransferase KAT2A, General Control of Amino Acid Synthesis Protein 5-like 2, Histone Acetyltransferase GCN5, HsGCN5, Lysine Acetyltransferase 2A, STAF97, GCN5L2, HGCN5)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到GCN5, Fluorogenic Assay Kit, BioAssay (KAT2A, Histone Acetyltransferase KAT2A, General Control of Amino Acid Synthesis Protein 5-like 2, Histone Acetyltransferase GCN5, HsGCN5, Lysine Acetyltransferase 2A, STAF97, GCN5L2, HGCN5)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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