This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Tumor hypoxia often directly correlates with aggressive phenotype, metastasis progression and resistance to chemotherapy. HIF-1 transcription factors are dramatically induced in hypoxic areas and regulate the expression of genes necessary for tumor adaptation to conditions of low oxygen. The stabilization of HIF-1a by hypoxia is critically dependent upon the hydroxylation of certain Proline residues that exist in the oxygen-dependent degradation domain of HIF-1a. HIF factors are now considered an important therapeutic target for cancer intervention. HIF-1a is useful to researchers interested in cell metabolism, cell survival, and angiogenesis.
应用类型
ELISA,Immunohistochemistry,Western Blot,
免疫原
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1a.
来源宿主
Rabbit
反应性
H. sapiens (Human)
保存建议
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
