中文名称

Monosodium Urate (MSU), crystals

英文名字

Monosodium Urate (MSU), crystals

供应商

USBiological

产品货号

045038

产品报价

￥1/2mg

产品说明书

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购买方式

银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868

产品新闻

背景资料

USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述

NLRP3/NALP3 inflammasome activator. Specially crystallized and tested for biological activity. Stimulates the caspase-1 activating NLRP3/NALP3 inflammasome, resulting in the production of active IL-1beta and IL-18.Appearance:Solid crystalsEndotoxin: ≤ 0.001EU/ug purified protein (LAL method)Solubility:Not soluble in PBS. Reconstitute 2mg of MSU in 100ul PBS to obtain a 20mg/ml suspension.Activation of THP-1 cells by uric acid:1. THP-1 cells are cultured in RPMI 1640 supplemented with 10% heat inactivated fetal bovineserum (FCS), 50uM B-mercaptoethanol, 2mM L-glutamine and penicillin/streptomycin. THP-1 cells are grown in suspension to a density of 1.5x10e6 cells/ml in tissue culture flasks.2. Treat THP-1 cells for 3 hours with 0.5uM PMA the day before stimulation.3. Wash cells with PBS and plate them in a 12-well plate at a density of 6x10e5 cells/well in RPMI medium.4. The next day, wash cells with PBS and add 500ul Optimem (Invitrogen).5. Stimulate the cells for 6 hours by addition of 50-300ug/ml of the suspension of uric acid (vortex the suspension just before use).6. As a positive control, 5mM of ATP is incubated for 6 hours.Detection of the mature Caspase-1 in the supernatant of uric acid-activated THP-1 cells:1. Collect the supernatant of each well (500ul). Add 500ul methanol and 125ul chloroform.2. Vortex and centrifuge for 5 min at 13,000 rpm.3. Remove the upper phase.4. Add 500ul methanol and centrifuge for 5 min at 13,000 rpm.5. Remove the supernatant, dry the pellet for 5 min at 50°C.6. Add 60ul of 1X loading buffer and DTT (final concentration of 0.1M).7. Mix and boil before loading on a 15% SDS-gel and transfer onto nitrocellulose membrane. The mature Caspase-1 is detected using the Caspase-1 (p20) (human), mAb (Bally-1) antibody (Cat No 044627).Storage and Stability:May be stored at 4ºC for short-term only. Long-term storage is recommended at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

产品形式

艾美捷向您提供的Monosodium Urate (MSU), crystals产品形式查看产品说明书，建议按照产品说明书进行应用操作。

保存建议

接收到Monosodium Urate (MSU), crystals产品后，置于-20°C温度下保存。

其他

Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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