- 中文名称
Insulin Like Growth Factor Binding Protein 3 (IGFBP3) BioAssay ELISA Kit (Rat)
- 英文名字
- Insulin Like Growth Factor Binding Protein 3 (IGFBP3) BioAssay ELISA Kit (Rat)
- 供应商
- USBiological
- 产品货号
- 025875
- 产品报价
- ￥1/48Tests ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Rat Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IGFBP3 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Detection Range:12.5-800ng/mlSensitivity:≤4.6ng/mlTest Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to rat IGFBP3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for rat IGFBP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IGFBP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IGFBP3 in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:*025875A: Microtiter Plate, 1x96 wells, Pre-coated; ready to use*025875B: Standard, 2x1vial 025875C: Standard Diluent, 1x20ml*025875D: Detection Reagent A, 1x120ul *025875E: Detection Reagent B, 1x120ul 025875F: Assay Diluent A, 1x12ml025875G: Assay Diluent B, 1x12ml025875H: TMB Substrate, 1x9ml*025875J: Positive Control, 231.8ng/ml 1x1vial025875K: Stop Solution, 1x6ml025875L: Wash Buffer, 30X, 1x20ml Precaution:The Stop Solution (025875K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Notes on 025875J: Positive Control:Intended Use:The control vial is from a rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.Characteristics:The control vial contains rat IGFBP3 in rat serum (lyophilized powder). Note: The rat based material used in this product is from healthy rats of SPF standard (specific pathogen-free animals).Expected Value:231.8ng/mlUsage:Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: After reconstitution, store Positive Control vial at 4°C and use within 5 days.Storage and Stability:Store *025875A, *025875B, *025875D, *025875E and *025875J at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
- 产品特点
- 针对Insulin Like Growth Factor Binding Protein 3 (IGFBP3) BioAssay ELISA Kit (Rat)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Insulin Like Growth Factor Binding Protein 3 (IGFBP3) BioAssay ELISA Kit (Rat)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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