95个肽段组成的肽池,来源于人类冠状病毒(HCoV)核蛋白(Nucleoprotein,瑞士蛋白库ID:P15130)的肽段扫描(15个氨基酸长度,11个氨基酸重叠)。
艾美捷PepMix HCoV-229E(NCAP)规格:
货号:PM-229E-NCAP-1
数量:每个肽段25 ?g(约15 nmol)(可用于刺激多达2.5×10?个细胞)
应用:T细胞免疫
类别:PepMix肽段池,PepMix感染相关肽段池
条件/主题:普通感冒,新冠肺炎(COVID-19),感染,呼吸道感染
布局:冷冻干燥于玻璃瓶中
生物体:人类冠状病毒(HCoV)
蛋白名称:核蛋白
纯度:研究级:每个肽段保证为主要产物(HPLC/MS)
定量:无
PepMix?肽段池:
覆盖整个蛋白的重叠肽段混合物(PepMix?)在以下应用中极为高效:
抗原特异性T细胞刺激:用于T细胞实验(例如ELISpot、细胞内细胞因子染色(ICS)、细胞介导的细胞毒性或增殖实验)
体外T细胞扩增
树突状细胞脉冲刺激
免疫监测
细胞免疫反应分析
T细胞表位鉴定
T细胞实验标准化
PepMix?的优势:
每个肽段均经过质量控制,并保证其身份和纯度
提供质量合格证(CoA)和HPLC-MS数据
批次间一致性高
由于严格纯化每个肽段,避免因污染缺失肽段导致的假阳性T细胞反应
由于严格纯化每个肽段,避免因毒素抑制T细胞反应
低生物负荷工艺,最小化内毒素污染
实施ADCF政策
HLA独立刺激(使用覆盖抗原的肽段池)
文献参考:
Peptide Tools to Support the Fight against COVID-19
Aude-Marie Alem1, Michael Drosch1, Ulf Reimer1, Pavlo Holenya1, Maren Eckey1, Florian Kern1,2, Holger Wenschuh1, Karsten Schnatbaum1
1 JPT Peptide Technologies GmbH, Volmerstrasse 5, 12489 Berlin, Germany. 2 Brighton and Sussex Medical School, Medical School Research
Building, Rm 109, Brighton BN1 9PS, East Sussex, UK.
To date, the understanding of the immune response to COVID-19 is still incomplete. In this application note we
describe innovative peptide-based tools for the assessment of humoral and cellular immunity to SARS-CoV-2.
These tools are suitable for immune response target identification and clinical immune monitoring. They can
also be used for T-cell and antibody response profiling and will be useful for the development of effective
diagnostics, treatments, and vaccines.
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