纯化的蛋白A,适用于ELISA、Western Blot、免疫沉淀和纯化实验。
作为SouthernBiotech在中国的区域总代理,艾美捷科技强烈推荐SouthernBiotech热销产品蛋白质A-无偶联。产品仅用于科研,不可用于临床诊断。
| 产品名称 | 货号 | 详情 |
蛋白质A-无偶联 | 7300-01S | 查看 |
蛋白质A-无偶联:
特异性:与人IgG1、IgG2和IgG4的Fc段结合,也与小鼠IgG2a和IgG2b结合;此外,还与兔、猪、豚鼠和犬的IgG结合。
来源:金黄色葡萄球菌
偶联物:UNLB(未偶联)
缓冲液配方:从蒸馏水中冻干
推荐储存条件:-20°C
操作和储存:纯化的(UNLB)蛋白A以5.0 mg或1.0 mg的纯化冻干蛋白形式提供。在复溶前,材料可在-20°C下储存长达一年。在PBS中复溶至尽可能高的浓度。复溶后,分装并储存于-20°C,最长可达六个月。
辣根过氧化物酶(HRP)偶联物以1.0 mL的形式提供,其母液为50%甘油/50% PBS,pH 7.4。未添加防腐剂。可在2-8°C下储存,或长期储存于-20°C。
如果按照指示储存,试剂在标签上标明的有效期内是稳定的。
应用:
ELISA – 经质量检测 1-7
纯化
Western Blot 8
免疫沉淀
文献参考:
1. Sondgeroth K, Blitvich B, Blair C, Terwee J, Junge R, Sauther M, et al. Assessing flavivirus, lentivirus, and herpesvirus exposure in free-ranging ringtailed lemurs in southwestern Madagascar. J Wildl Dis. 2007;43:40-7. (ELISA)
2. Wycoff KL, Belle A, Deppe D, Schaefer L, Maclean JM, Haase S, et al. Recombinant anthrax toxin receptor-Fc fusion proteins produced in plants
protect rabbits against inhalational anthrax. Antimicrob Agents Chemother. 2011;55:132-9. (ELISA)
3. Duthie MS, Truman RW, Goto W, O'Donnell J, Hay MN, Spencer JS, et al. Insight toward early diagnosis of leprosy through analysis of the developing
antibody responses of Mycobacterium leprae-infected armadillos. Clin Vaccine Immunol. 2011;18:254-9. (ELISA)
4. Arzola L, Chen J, Rattanaporn K, Maclean JM, McDonald KA. Transient co-expression of post-transcriptional gene silencing suppressors for
increased in planta expression of a recombinant anthrax receptor fusion protein. Int J Mol Sci. 2011;12:4975-90. (ELISA)
5. Karuppanan K, Duhra-Gill S, Kailemia MJ, Phu ML, Lebrilla CB, Dandekar AM, et al. Expression, purification, and biophysical characterization of a
secreted anthrax decoy fusion protein in Nicotiana benthamiana. Int J Mol Sci. 2017;18:89. (ELISA)
6. Xiong Y, Li Q, Kailemia MJ, Lebrilla CB, Nandi S, McDonald KA. Glycoform modification of secreted recombinant glycoproteins through kifunensine
addition during transient vacuum agroinfiltration. Int J Mol Sci. 2018;19:890. (ELISA)
7. Sukenik SC, Karuppanan K, Li Q, Lebrilla CB, Nandi S, McDonald KA. Transient recombinant protein production in glycoengineered Nicotiana
benthamiana cell suspension culture. Int J Mol Sci. 2018;19:1205. (ELISA)
8. Naskar P, Puri N. Phosphorylation of SNAP-23 regulates its dynamic membrane association during mast cell exocytosis. Biol Open. 2017;6:1257-69.(WB)
SouthernBiotech(SBA)公司建于1982年,致力于为免疫研究的科学家提供各种亲和纯化的多克隆和单克隆二抗和其它特异性抗体,其中包括:荧光素和酶标记物,低内毒素/不含叠氮化物制剂,F(ab)和F(ab’)299片段化抗体,此外还有纯化的免疫球蛋白、血清以及细胞外基质蛋白等产品。作为SouthernBiotech在中国的区域总代理,艾美捷科技有限公司将为中国客户提供全面的SouthernBiotech产品以及客户订制化服务。

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