高灵敏度BTLA:HVEM[生物素化]抑制剂筛选检测试剂盒

BTLA 与 HVEM 受体（疱疹病毒进入介质，也称为 TNFRSF14）的结合触发参与 T 细胞负调节的细胞信号传导途径。BTLA:HVEM 相互作用在炎症、自身免疫和抗肿瘤反应的调节中发挥着关键作用，并且是药物发现的重要靶标。BTLA:HVEM[生物素化]抑制剂筛选检测试剂盒专为筛选和分析 BTLA:HVEM 信号传导抑制剂而设计。该试剂盒的关键在于链霉亲和素-HRP 检测生物素标记的 HVEM 的高灵敏度。该测定只需要在微量滴定板上进行几个简单的步骤。首先，将 BTLA 涂覆在 96 孔板上。接下来，HVEM 与 BTLA 在板上一起孵育。最后，用链霉亲和素-HRP 处理板，然后添加 HRP 底物以产生化学发光。

艾美捷 BTLA:HVEM[生物素化]抑制剂筛选检测试剂盒：

货号：72008

英文名称：BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit

规格：96 rxns.

同义词：CD272、CD270、TNFRSF14、ATAR、HVEA、LIGHTR、TR2、活性检测试剂盒、Strep HRP、链霉亲和素

检测试剂盒格式：化学发光

提供方式：该试剂盒采用方便的 96 孔格式，配有生物素标记的 HVEM、纯化的 BTLA、链霉亲和素标记的 HRP 以及可进行 100 次结合反应的测定缓冲液。

科学类：细胞表面受体/单次跨膜蛋白

储存/稳定性：按指示保存时，自收到之日起 6 个月。

应用领域：该试剂盒可用于筛选 HVEM 与 BTLA 结合的抑制剂。

运输温度：-80°C（干冰），避免冷冻/解冻循环。

需要但未提供的材料：

PBS（磷酸盐缓冲盐水）

发光计或酶标仪能够读取化学发光

旋转或摇杆平台

成分：

BTLA:HVEM[生物素化]抑制剂筛选检测试剂盒文献参考：

1. Derre L., et al. J. Clin. Invest. 2010, 120(1): 157-167.

2. Steinberg, M.W., et al. J. Exp.Med. 2008, 205(6): 1463-1476.

部分引文参考：

1、Structural and Biochemical Analysis of the Dual Inhibition of MG-132 against SARS-CoV-2 Main Protease (Mpro/3CLpro) and Human Cathepsin-L

Elisa Costanzi, Maria Kuzikov

International Journal of Molecular Scienc…    |    2021 Oct 29    |    PubMed ID: 34769210    |    Read Article  

"We have measured no activity towards the second SARS-CoV-2 protease PLpro (paper in preparation).. Looking at dose-dependent MG-132 inhibition of Cathepsin-L using the BPSBioscience inhibitor screening kit, we saw a strong effect with 0.15 nM IC50 which corresponded to half of the used enzyme concentration in this biochemical assay ( ).. Previously, the inhibition of rat liver isolated Cathepsin-L was reported to be in the .."

2、Screening of Clinically Approved and Investigation Drugs as Potential Inhibitors of SARS‐CoV‐2: A Combined in silico and in?vitro Study

SerdarSerdar Durdagi Durdagi, Muge Didem Orhan

Molecular Informatics    |    2021 Sep 16    |    PubMed ID: 34529322    |    Read Article  

".. and rotigaptide) for the Spike/ACE2 interactions were ordered and tested by in vitro experiments.. Two different inhibitor screening assay kits against Spike protein were used as ACE2:SARS‐CoV‐2 Spike S1 (#79945) and SARS‐CoV‐2 Spike:ACE2 (Cat. #79931), (BPS Bioscience).. Both assays were designed for screening and profiling inhibitors of the Spike and ACE2 .."

3、Triple-target stimuli-responsive anti-COVID-19 face mask with physiological virus-inactivating agents

Werner E. G.Werner E. G. Müller Müller, MeikMeik Neufurth Neufurth

Biomaterials Science    |    2021 Jun 30    |    PubMed ID: 34190748    |    Read Article  

"A Screening Assay Kit (BPS Bioscience/Tebu-bio, Offenbach; Germany) was used as described.. In this system the recombinant ACE2 receptor (50 ng per well) was bound to .."

BPS bioscience总部坐落于世界最大之一的美国圣地亚哥生命科学研究机构和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、组蛋白去甲基化酶、组蛋白甲基化转移酶、HDACs、聚ADP核糖聚合酶。BPS公司为药物研发用重组酶类的提供了最大选择之一。