Jena Bioscience：脂质立方相（LCP）结晶

脂质立方相（LCP）结晶已经发展成为膜蛋白结晶的一种重要方法，其中脂质单油酸甘油酯（Monoolein）是创建稳定LCP的首选。然而，在过去几年中，短链脂质7.7 MAG^[4,7]、7.8 MAG^[6-8]和7.9 MAG^[5,7]变得越来越受欢迎。最近在将高黏度脂质立方相应用于连续飞秒晶体学（LCP-SFX）方面的进展^[1-3]，通过以下方式进一步加速了LCP方法的成功：

• 将载有晶体的黏性LCP直接送入X射线自由电子激光（XFEL）光束中（与液体注射器相比，减少了样品消耗)；

• 利用在LCP中生长的固有小晶体；

• 避免了从LCP中进行棘手的晶体装载。

JBScreen LCP旨在高效筛选LCP中的结晶条件。上述表格中的每种LCP脂质都能创建一个稳定的脂质立方相，可用于与JBScreen LCP进行结晶设置。其96种条件是通过对192种成功通过in meso方法结晶并产生结构的整合膜蛋白进行数据挖掘得出的^[7]。该筛选按沉淀剂的浓度和类型排序，并且不含乙酸镉。

文献：

[1] Nogly et al. (2016) Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography. Nat. Commun. 7:12314.

[2] Zhu et al. (2016) Serial Femtosecond Crystallography of Membrane Proteins. Adv. Exp. Med. Biol. 922:151.

[3] Batyuk et al. (2016) Native phasing of x-ray free-electron laser data for a G protein-coupled receptor. Sci. Adv. 2:e1600292.

[4] Coincon et al. (2016) Crystal structures reveal the molecular basis of ion translocation in sodium/proton antiporters. Nat. Struct. Mol. Biol. 23:248.

[5] Li et al. (2015) Ternary structure reveals mechanism of a membrane diacylglycerol kinase. Nat. Commun. 6:10140.

[6] Fowler et al. (2015) Gating Topology of the Proton-Coupled Oligopeptide Symporters. Structure 23:290.

[7] Caffrey (2015) A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes. Acta Cryst F 71:3.

[8] Li et al. (2013) Crystal structure of the integral membrane diacylglycerol kinase. Nature 497:521.

[9] Misquitta et al. (2004) Rational design of lipid for membrane protein crystallization. Journal of Structural Biology 148:169.