首先，将Nε-(羧乙基)赖氨酸（CEL）偶联物包被在ELISA板上。然后，将未知的CEL蛋白样本或CEL-牛血清白蛋白（BSA）标准品加入到预包被有CEL偶联物的板中。短暂孵育后，加入抗CEL单克隆抗体，随后加入辣根过氧化物酶（HRP）标记的二抗。未知样本中CEL蛋白加合物的含量通过与预先确定的CEL-BSA标准曲线进行比较来确定。尽管CEL和Nε-(羧甲基)赖氨酸（CML）在结构上相似，但OxiSelect™ CEL ELISA试剂盒中的抗CEL特异性抗体不会与CML蛋白加合物发生交叉反应。 First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation, the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the predetermined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in the OxiSelect™ CEL ELISA Kit will not cross react with CML protein adducts.
