超氧化物歧化酶（SOD）可催化超氧阴离子歧化为过氧化氢和分子氧，是最重要的抗氧化酶之一。SOD酶可分为三类：胞质铜锌超氧化物歧化酶（CuZn-SOD）、线粒体锰超氧化物歧化酶（Mn-SOD）和细胞外超氧化物歧化酶（Ec-SOD）。我们的OxiSelect™超氧化物歧化酶活性检测试剂盒使用黄嘌呤/黄嘌呤氧化酶（XOD）系统生成超氧阴离子，并使用一种显色剂，在被超氧阴离子还原时生成一种水溶性的甲臜染料。超氧化物歧化酶的活性通过对显色剂的抑制或还原程度来确定。 Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidant enzymes. SOD enzymes are classified into three groups: cytosolic CuZn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect™ Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions and a chromagen to produce a water-soluble formazan dye upon reduction by superoxide anions. The superoxide dismutase activity is determined as the inhibition or reduction of chromagen.
