小鼠来源单克隆抗体：Amyloid beta peptide（A-beta 40/42），【MOAB-2】 - Biotinylated-Mouse monoclonal antibody to Amyloid beta peptide (A-beta 40/42),【MOAB-2]

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中文名称: 小鼠来源单克隆抗体：Amyloid beta peptide（A-beta 40/42），【MOAB-2】 - Biotinylated

英文名字: Mouse monoclonal antibody to Amyloid beta peptide (A-beta 40/42),【MOAB-2] - Biotinylated

供应商: Biosensis

产品货号: M-1742-50-B

产品报价: ￥询价/50ug

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应用类型: WB; IH, Frozen; IF,Frozen; IH(P), Paraffin embedded; IF; IP; ELISA
The biotinylated MOAB-2 antibody has been tested by IHC (1:500 - 1:2,000 dilution) and is also expected to work in applications validated for the unlabelled antibody (M-1586-100) at same or higher dilutions: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry/paraffin embedded IHC(P), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA.

Western Blotting:

MOAB-2 has been tested in WB using purified synthetic beta-amyloid preparations and from transgenic mouse brain formic acid extracts (see Figure 1). Formic acid extraction/concentration is required for western blot detection from extracts. Suggested dilution of 1:2000-1:5,000 for WB, standard ECL detection systems.

Tissue samples for the detection of beta-amyloid should be prepared as detailed in Youmans KL et al., 2011 (Journal of Neuroscience Methods 196: 51-59) for best results. Detection of beta-amyloid 40/42 in direct westerns can be difficult; Dot-blots of prepared samples are recommended as detailed in Youmans KL et al., 2012.

Immunohistochemistry:

Suggested dilution for biotinylated MOAB-2 in IHC is 1:500-1:2,000. Fresh frozen, 4% paraformaldehyde fixed frozen, or formalin fixed paraffin embedded tissues are all suitable. Antigen retrieval is required in fixed tissues for optimal staining.

Antibody was tested on 4% paraformaldehyde/0.1% glutaraldehyde fixed frozen tissue from 3xTg and 5xFAD mice. MOAB-2 antibody detects intraneuronal and extracellular beta-amyloid in IHC and does not detect APP (Youmans KL et al., 2012).

The antibody also reacts with archival formalin-fixed, paraffin-embedded tissue samples with antigen Heat Induced Epitope Retrieval (HIER). Recommended buffer for HIER is citrate, pH 6.0. Signal was weak without antigen retrieval. Immunoreactivity was observed in intraneural-amyloid deposition (plaque) in Alzheimer's brain. MOAB-2 was found to be extremely clean and with an excellent signal to noise ratio with no neuro-cellular diffusive staining.

In addition, MOAB-2 demonstrated no significant differences in A-beta detection using paraffin fixed, free-floating sections (Youmans KL et al., 2012). Formic acid (FA) treatment resulted in optimal detection of both intraneuronal and extracellular A-beta compared to without FA (incubated in 88% FA 8 min, Youmans KL et al., 2012). Free floating tissue sections were permeabilized in TBS containing 0.25% Triton X-100 (TBSX; 3 x 10 min), blocked with 3% horse serum in TBSX (3 x 10 min) followed by 1% horse serum in TBSX (2 x10 min) and incubated with appropriate primary antibodies diluted in TBSX containing 1% horse serum overnight. See Youmans KL et al., 2012, for full IHC(P) protocol and method details.

Immunofluorescence:

For IF, suggested dilution is 1:100-1:500. The antibody was tested on 4% PFA fixed frozen tissue. Fixed tissues were washed in TBS (3 x 10 min), then incubated in 88% FA (8 min), and then permeabilized in TBSX (3 x 10 min), and blocked in TBSX containing 5% bovine serum albumin (BSA; 1 hr). Sections were subsequently incubated with appropriate primary antibodies diluted in TBSX containing 2% BSA overnight on an oscillatory rotator. Detection was via fluorescently labelled absorbed secondary antibodies (Youmans KL et al., 2012).

Immunoprecipitation:

For IP, the suggested dilution is 1:200 to 1:1,000 for labelled beta-amyloid using SA-coated beads as the capture vehicle, similar to the protocols employed by Youmans KL et al., 2012.

ELISA:

In an ELISA, a dilution of 1:50-1:1,000 is suggested. The antibody has been tested in ELISAs on synthetic beta-amyloid and tissue homogenates from beta-amyloid-Tg mice.

Biosensis recommends optimal dilutions/concentrations should be determined by the end user for all applications. Dilutions provided are only meant to serve as a basic guide.

免疫原: Recombinant human amyloid beta protein 42 (AB42): DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

来源宿主: 小鼠

反应性: 人, 大鼠, other species not yet tested. By Dot Blot, MOAB-2 detected 大鼠 AB40, 人 AB40, albeit with less affinity than for AB42 (Youmans KL et al., 2012).

保存建议: 厂家建议常温运输。抗体溶解之后，建议分装保存于-20℃或-70℃。在2-8 ℃可保存1周（避光，使用无菌器材）。按照1:1体积比添加最高纯度的甘油，可增加抗体的稳定性。请避免反复冻融。不适用时，应保持盖子紧闭并避光保存。

其他: Biosensis专注于神经科学领域的抗体和试剂的开发，特别是神经营养因子和神经营养因子受体。近30年来，Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子，我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症（AD）、帕金森氏症（PD）和肌萎缩侧索硬化（ALS）疾病，以及自噬和代谢应激障碍，包括肥胖、代谢综合征的研究、神经免疫学和炎症。Biosensis的产品系列不仅包括持续增长的神经科学研究抗体系列，还包括200多种定量研究ELISAs试剂盒（1板或2板，自由选择），组织染色（退化神经元染色试剂盒、病理髓鞘染色试剂盒、淀粉样斑块染色试剂等）和细胞可视化试剂（染色）以及针对神经科学和细胞疾病研究的纯化的蛋白质。我们的抗体和试剂已被广泛应用于各种技术，包括蛋白质印迹，免疫组织化学，流式分析，共聚焦显微镜实时成像，生物抑制和细胞培养以及克隆。